techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for separating a methylene blue/sodium fluorescein mixture by thin layer chromatography using silica gel and alumina as the stationary
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community with 3 locations in central Sydney‚ Epping and Granville and was founded in 2002. Dance Kool is the pioneer of dance communities within New South Wales region that aims to unite people from different backgrounds to participate in recreational activity. The multiculturalism embedded within this community does not tolerate prejudice‚ discrimination and exclusive attitudes. The Core values of Dance Kool are the encouragement of growth in terms of craftsmanship and freedom of expression but also
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separated by a procedure called chromatography. The procedure uses a special paper and solvent. The chlorophyll molecules adhere to the paper. The solvent molecules move up the paper by capillary action. Each chlorophyll molecule will travel up the paper at different rates. Hypothesis: If chlorophyll is removed from the plant‚ then the different pigments in the plant can be visible. Materials: -Spinach -Coin -Chromatography paper -Ruler
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Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
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PAPER and COLUMN CHROMATOGRAPHY REPORT 1. 2. The unknown code is an amino acid‚ Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine 3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase‚ the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
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packed closely together‚ but they are able to move around. Hence a fluid does not have a rigid shape‚ but adapts to the contours of the container that holds it. Like a liquid a gas has no fixed shape‚ but it has little resistance to compression because there is enough empty space for the molecules to move closer. Whereas a liquid placed in a container will form a puddle at the bottom of the container‚ a gas will expand to fill the container. Although the three categories seem very well defined
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39. The Viscosity of Liquids After studying the present lecture‚ you will be able to Define viscosity and viscosity coefficient Outline the method to measure viscosity using Ostwald viscometer Determine the average molecular weight of a polymer Determine the surface concentration of 1-butanol in aqueous solution Measure the distribution coefficient of a solute betweenn two solvents 39.1 Introduction Viscosity‚ one of the transport properties‚ arises because of intermolecular attractive and
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________________________12345_______________________ Date ____23.8.2013___________ Block _______ Training Lab: Paper Chromatography I. Background Information: When working in a lab‚ scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First‚ you will separate the unknown chemicals and then you will identify
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Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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of solvent extraction‚ also known as liquid-liquid extraction‚ a method of separating two materials with different physical and/or chemical properties. Two methods of evaporation will be used to finally determine the concentration of impurity- one using an explosion-proof hot-plate‚ and one using a rotovap. III. Theory Fig. 1a+1b. Figure 1a shows how water and oil separate in the separatory funnel‚ while figure 1b shows the actual apparatus. Liquid-Liquid extraction is a versatile and dependable
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