Experiment 4 – Liquid Phase Chromatography I. Objectives This experiment’s goal is to explore one-dimensional and two-dimensional paper chromatography. II. Schematic Diagram of the Procedure PAPER CHROMATOGRAPHY Wash leaves‚ cut them into smaller pieces; in a mortar macerate them in circular motion Add 8mL ethyl alcohol to extract pigments‚ continue macerating until finely grounded Transfer extract to evaporating dish‚ allow to conc‚ don’t let extract to dry out Concentration
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC)
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Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Explanation HOW TO COLOR YOUR HAIR WITH KOOL-AID I am going to teach you how to color your hair by using Kool-Aid. Make sure when using Kool-Aid‚ you don’t use the pre-sweetened Kool-Aid‚ for it will make the hair very sticky. First‚ I am going to have you put on gloves to prevent staining. If you don’t want to wear gloves‚ be aware that your skin may stain but that you can still remove the Kool-Aid from it and I will have the directions on how to remove the Kool-Aid from the skin at the end of these
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How to make Kool-Aid There are five simple steps on how to create this candy tasting drink. Choosing the proper packet of flavoring is the first step in making Kool-Aid. Check your local grocery store’s shelf for a variety of flavors‚ from Mountain Berry Punch to Tropical Blue Hawaiian. They are very cheap‚ usually under 65 cents. After choosing the flavor that suits you‚ the second step is making sure you have the correct and necessary equipment for making Kool-Aid. You will need a two-quart pitcher
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other people though we do not realize the effects that it will have on culture. The effect of the medium becomes the blue print and builds integration to society. These effects are McLuhan’s message. In Tom Wolfe’s non-fiction novel‚ “Electric Kool-Aid Acid
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Bio IH – Test Study Guide Ecology Be able to answer the following questions THOROUGHLY: 1. Identify the different levels of organization that ecologists study. 2. Give examples of populations‚ communities‚ ecosystems‚ biomes‚ niches‚ habitats and their interactions within an ecosystem. 3. Be able to trace the flow of energy through living systems. 4. Evaluate the efficiency of energy transfer among organisms in an ecosystem. 5. Compare food webs and food chains. 6. Describe why food chains
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English Book Report The Electric Kool-Aid Acid Test The Electric Kool-Aid Acid Test is a novel about a the exploration of drug use and the many effects drug use can have on ones life. Ken Kesey‚ the main character volunteered to participate in a drug study advised by the CIA. Ken Kesey is an intelligent young journalist who attends Stanford University‚ and participates in the Creative Writing Program. Ken moves to California‚ where he shares a house with a group called the "Merry
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Experiment 5 Analysis of Analgesic Tablet by High Performance Liquid Chromatography Abstract An unknown sample‚ 529‚ was tested using high performance liquid chromatography to detect the concentrations of acetaminophen‚ aspirin‚ and caffeine respectively. There was found to be 4.03±0.144mg/100mL of acetaminophen‚ 11.5±0.185mg/100mL of aspirin‚ and 4.89±0.185mg/100mL of caffeine. Based on accepted values‚ the maximum daily amounts of each compound are 4000mg of acetaminophen
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COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos‚ Kuia B. Allarde‚ Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment‚ the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to
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