Protocol Materials: * Fertilized chicken eggs (Gallus gallus) * Tumor cell line (SK-ChA-1) * 70% ethanol * Paper towel * 45 Petri dishes * Incubator * Non-fertilized eggs * Rocket fuel * Marker * Scalpels * PBS-suspension * Fine forceps * Decapitation-scissors * Plastic rings 1mm diameter * 80 mm triangular magnetic stir bar * DCCP + DSPE-PEG 96:4 mmol empty liposomes suspension * DCCP + DSPE-PEG 96:4 mmol + Zn-Phthalocyanine (Lipid:PS
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Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday‚ September 18‚ 2008OBJECTIVESIn this lab experiment two different types of bacteria‚ Escherichia coli and Staphylococcus aureus‚ were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies. Resulting data was analyzed to provide understanding of the use of differing culture media and conditions for bacterial growth. RESULTSFour different agar types were used in this
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Techniques for Isolation of Pure Cultures Objective : i. To perform the spread plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate
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Table 1. Observations from week 2 for the detection of ammonia using the Nessler’s reagent and from week 1 for the pH using bromothymol blue indicator with the inoculation of P. vulgaris‚ P. fluorescens‚ and B. Cereus in peptone broth. Tubes were incubated at room temperature for 7 days and 14 days. Soil Microorganism Nessler’s Reagent (color reaction pH (bromothymol blue) Our results pH (bromothymol blue) Class results P. vulgaris Deep yellow ++ 8.0 8.0‚ 7.5‚ 6-7‚ 11.5 P. fluorescens
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3.3 Microbiological Analysis 3.3.1 Bacteria count using Heamocytometer This method was used to determine the of three types of dessert but does not differentiate the type of bacteria. 25 g of food samples will be homoginesed in a sterile stomacher bag and shaken for two minutes with 225 ml of peptone water to obtain the food mixture. Using separate sterile pipets‚ decimal dilutions of 10-2‚ 10-3‚ 10-4‚ 10-5 will be prepared and others as appropriate‚ of food homogenate by transferring 10 ml of previous
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Lab‚ Week ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY Introduction Part I: Aseptic Technique The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique‚ which is used to avoid contaminating cultures. In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining
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Impatiens balsamina Linn. (Kamantigue) Flower Extract: A Potential Antifungal Agent for Candida albicans and Trichophyton mentagrophytes A Thesis Presented to the Faculty of the College of Medical Technology Unciano Colleges Inc-Antipolo In Partial Fulfillment Of the Requirements for the Degree Bachelor of Science in Medical Technology By: Bernardo‚ Reynalyn Cruz‚ Nor Rizsellito Lagman‚ Joyce Anne Leonor‚ Zheidi Ann Mari Samson‚ Danica November 2012 ABSTRACT This study was conducted to determine
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obtain two microtubes and label one +pGlo and the other –pGlo with a marker. Next‚ the tubes were placed in the microtube rack and 250 μl of CaCl2 was transferred into each tube using a sterile pipette. After placing the microtubes in ice‚ a sterile inoculation loop was used to pick up a single colony of bacteria from the E. coli starter plate. Next‚ the +pGlo microtube was removed from the ice and‚ to ensure that the colony was efficiently dispersed into the
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ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and
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systemic hosts provide another advantage for genomic and quantitative studies. As shown in Fig. 1.3‚ CMV particles reach a high accumulation condition within four days of inoculation in cells of the inoculated leaf. And the potyvirus infection also shows distinguished characteristics. Fig. 1.2. Typical symptoms caused by inoculation of Cucumber mosaic virus (a) Nicotiana glutinosa‚ presenting irregular yellowing spots on the inoculated leaves; (b) Chenopodiu
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