Figure 3.1: Typical standard chromatogram of Ritonavir and Lopinavir Figure 3.2: Typical sample chromatogram of Ritonavir and Lopinavir 3.3.2 Method validation The optimized RP-HPLC method for simultaneous assay of Ritonavir and Lopinavir was validated according to ICH guidelines with respect to system suitability‚ linearity‚ sensitivity‚ accuracy‚ precision and robustness. 3.3.3 System suitability Before sample analysis‚ the chromatographic parameters used in this analysis must conform the system
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Tiboelectric Series Lab Fleur Vande Kerckhove 9A Purpose/Aim: How do the triboelectric series from different materials classify themselves due to their affinity (gaining or losing) when being measured by a gold leaf electroscope? Hypothesis: If the materials change (from: wool‚ rabbit fur‚ cotton‚ aluminum‚ nylon‚ amber‚ celluloid‚ silver‚ silicon and acrylic) to classify their affinity of their triboelectric series then rabbit fur will have a higher affinity compared to the rest of the materials
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Lab Report Format for Separating the Components of “Panacetin” (2) and Recrystallization and Melting Point Measurement: Identifying the Components of “Panacetin” (3). Lab Notebook Title and Date Objective: Explain the purpose of the experiment and how you plan to accomplish it. Technique: Show the use of separatory funnel and evaporation of solvent setup Reaction(s): Include the main reaction for the experiment and isolation scheme Physical Data: List the molecular weight‚ melting point‚ boiling
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9.1 Orbitals and Theories of Chemical Bonding 1. Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct? a) MO theory predicts that electrons are localized between pairs of atoms. b) In VB theory‚ bonding electrons are delocalized over the molecule. c) MO theory accurately describes bonding in O2 and NO‚ VB theory does not. d) VB theory can describe molecular bonding in excited states. e) MO theory is used to accurately predict
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Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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Lab 5 Column Chromatography: Isolation of Lycopene from Tomato Paste Reading: Zubrick‚ pages 79-82‚ 127-130‚ 138-139‚ 141-143‚ and 235-240 Pre-lab: look up the structure of lycopene. Introduction: Lycopene is the red pigment in ripe tomatoes and‚ as an antioxidant‚ helps to fight certain cancers. In this lab you will isolate lycopene from tomato paste. To do this you will first extract carotenoid pigments from the paste and then use column chromatography to isolate the lycopene from
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Column and thin layer chromatography of plant pigments CHEMISTRY 201L EXPT 04 PAGE 01 - 12 Noel Angelo P. Kalacas*‚ Hanna Mae Laluces‚ Ina Bianca Lanuza Department of Chemistry‚ College of Science *Corresponding author; e-mail: knight_BeNcH66@yahoo.com Abstract Chromatography is a powerful technique for separating and/or identifying the components in a mixture. There are different types of chromatography and each has its own strengths and weaknesses. In this experiment‚ pigments of the
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A simple‚ rapid‚ specific and highly sensitive spectrofluorimetric method has been developed for the quantification of dabigatran etexilate mesylate (DAB) in bulk and capsule dosage form. A linear relationship was found between fluorescence intensity and DAB concentration in the range of 0.01-1.0 μg/ml in DMSO as solvent at an emission wavelength of 391 nm after excitation at 334 nm‚ with a good correlation coefficient (0.989). The detection and quantification limits were found to be 0.005 and 0
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Name _____________________________ Date ____________ Period _______ Completing the Square Day 1 For each expression‚ find the number you would add to make it a perfect square trinomial. Leave fraction answers as improper fractions (no mixed numbers or decimals). Then factor each trinomial. 1. x2 + 10x + _______ 2. y2 – 6y + ¬¬¬¬_______ 3. z2 – 8z + _______ _____________________ _____________________ _____________________ 4. x2 + 12x + _______ 5. x2 – x + _______ 6.
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Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
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