Description: A peroxidase enzyme‚ which was extracted from a brassica compestris (turnip)‚ is tested under various conditions in temperature‚ pH level‚ and competitive inhibitor (hydroxylamine). ABSTRACT: In order to determine the properties of an enzyme‚ a peroxidase enzyme was extracted from a brassica compestris (turnip) and tested under various temperatures‚ pH levels‚ and by a competitive inhibitor (hydroxylamine). The enzyme activity was measured in various ways depending on the activity
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Potato Enzyme Lab INTRODUCTION An enzyme is a protein that speeds up or slows down a specific chemical reaction in an organism. A good rule of thumb is to remember that enzyme names end in “-ase”. This will help in identifying enzymes in further readings. Generally enzymes are catalysts. Hydrogen peroxide is a toxic chemical that is produced in many organisms during metabolism. Organisms must get rid of this toxin to survive. One reaction turns the hydrogen peroxide into water and oxygen. The
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Cavanaugh Lab: Tuesdays at 1pm Enzyme Kinetics Lab Introduction: Enzymes are proteins that will catalyze reactions to make the rate of the reaction occur faster than it would without. It can also make the reaction occur in the first place. Tyrosinase is an enzyme that has a variety of functions and activities. It produces pigments like melanin and others that would be apparent when a fruit is cut in half and it browns. (Bien-etre 3).There is that one function that stands out and the enzyme is continuously
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Investigation on the effects of Temperature‚ pH levels‚ and Enzyme Concentration on the reaction rate of the Decomposition of Hydrogen Peroxide by Catalase The purpose of this investigation is to figure out how temperature‚ pH‚ and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to
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In Graph 1‚ each line of color represents a different level of pH added to the solution. When pH 9 was added‚ it produced the highest rate of reaction (the most oxygen was produced)‚ whereas when the more acidic pH 5 was added‚ the rate of reaction was much slower‚ and less efficient. The higher the oxygen evolved (gas produced)‚ the more reactions were being produced/higher rate of reaction. Different types of enzymes’ reaction proportions differ based on the pH being added. For example‚ intestinal
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Title: The Effect of Varying Amounts of Substrate and Enzyme on a Reaction Rate Abstract In living organisms‚ certain reactions must take place rapidly to assist life. This occurs because of enzymes‚ because all reactions would take place too slowly to sustain life (Jacklet‚ 237). Enzymes are large protein molecules that catalyze specific chemical reactions without being used up in the process. Each enzyme has a region on its surface‚ called the active site‚ which recognizes a specific
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Purpose: The purpose of this lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will
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Data Analysis: Graph 1 indicates the relationship between the dependent and the independent variable to be; as the concentration of sodium bicarbonate in the solution submerging the leaf discs is increased‚ the average rate of photosynthesis of the leaf discs increased in a linear trend. As it is the sodium bicarbonate which decomposes into carbon dioxide necessary for photosynthesis‚ it is reasonable to consider from Graph 1 that as carbon dioxide concentration of the solution increases‚ the rate
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concentrations; that test the reaction rates of the enzyme catalase over a fixed period of time. The major conclusion was that catalase reacts faster in warm temperatures that are neither freezing nor boiling‚ catalase performs well in lower concentrations than the substrate‚ and catalase prefers neutral pH levels around 7. Introduction Enzymes are proteins that catalyze metabolic reactions vital for the survival and functioning of cells [1]. Without enzymes‚ metabolic processes would occur at unfeasible
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Sterling Hayden AP Biology Mrs. Proehl 24 November 2014 I. Title Effect of Differing pH‚ Temperature‚ and Enzyme Concentration on Catalase Reaction Rate II. Introduction Enzymes are used to increase the rate of specific reactions in the body. Catalase‚ a specific enzyme‚ speeds the breakdown of hydrogen peroxide‚ a toxic chemical produced by cells in the body‚ into water and oxygen (Cain and others‚ 2010). The oxygen can be observed as bubbles coming from the reaction site. Catalase is found in many living tissues of organisms
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