Preview

Enzyme Catalysis Lab

Satisfactory Essays
Open Document
Open Document
280 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Enzyme Catalysis Lab
Purpose:

The purpose of this lab was to observe and understand the effects of changes in temperature, pH, enzyme concentration, and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions.

Hypothesis:

I believe that if there is an increase in enzyme concentration, an increase in temperature, or an increase in pH, then the intensity of the reaction will increase, which will cause greater alterations in the product of the reaction. I think this because when there is an increase in temperature or pH substrates collide with active sites more frequently due to molecules moving rapidly.

Materials:

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Purpose: The purpose of this lab is to demonstrate how temperature, substrate concentration, and pH can impact the rate of an enzyme reaction.…

    • 704 Words
    • 8 Pages
    Satisfactory Essays
  • Better Essays

    Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.…

    • 2040 Words
    • 9 Pages
    Better Essays
  • Good Essays

    Hold in liquid for about three-seconds and then touch the disk to the inside of the beaker to remove excess drops of liquid.…

    • 591 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Enzyme Lab Quiz

    • 293 Words
    • 2 Pages

    a. Temperature affects the rate at which substrate and enzyme molecules collide. If the temperature is greater than the optimal the activation site denatures which makes binding more difficult. Lower temps make it so that the enzymes and substrates attach at a slower rate, diminishing product formation.…

    • 293 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Enzyme Lab Report

    • 1060 Words
    • 5 Pages

    The increasing temperature increases molecular motion and may increase the number of times an enzyme contacts and combines with a substrate molecule. Temperature may also influence the shape of the enzyme molecule, making it fit better with the substrate.…

    • 1060 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Enzyme Lab

    • 1242 Words
    • 6 Pages

    Title: The Effect of Adjusted Concentration of Hydrogen Peroxide on the change in reaction rate of liver catalase.…

    • 1242 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Lab Report Enzyme Lab

    • 743 Words
    • 3 Pages

    Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature, pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions, which would otherwise progress more slowly.(Background Information; pg. 1) pH is a measurement of the acidity or alkalinity (base) of a solution. When the liver got mixed with H2O2 , the first time the chemical reaction was fast, the second time the reaction was slow and the last try was very fast. Temperature is the degree or intensity of heat present in a substance or object. When the temperature of the liver changed from freezing to very hot to room…

    • 743 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Enzyme Lab Report

    • 1528 Words
    • 5 Pages

    experiment is to determine what changes in pH, temperature, and enzyme concentration have on the rate…

    • 1528 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Ap Biology Enzyme

    • 425 Words
    • 2 Pages

    1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions, such as, different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with, 1.5 ml of H2O2, 1.5ml of H2O and different amounts of enzyme drops, to determine how much oxygen gas is produced during the reaction since the pressure of the test tube will get higher as more oxygen gas is accumulated during the reaction.…

    • 425 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Lab 6 enzymes

    • 1000 Words
    • 5 Pages

    Purpose: The purpose of this lab is to test for enzyme activity, look at enzyme specificity, and how temperature affects enzyme activity.…

    • 1000 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    Enzyme Lab

    • 1094 Words
    • 5 Pages

    This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased, then the reaction rate would also increase. To test our question, we varied a combination of substrate and buffer, totaling 6mL, with a constant amount of 2 drops of catalyst. The enzyme catalyst, peroxidase, increased the rate of the reaction. The results of our experiment can be found by comparing the reaction rates for each trial. These rates are actually the slopes of the lines that were graphed during each reaction. The trend of data…

    • 1094 Words
    • 5 Pages
    Powerful Essays
  • Powerful Essays

    Introduction: This lab tested how enzymes are able to affect the rate of chemical reactions and how the rate of which an enzyme works in different conditions. The conditions the enzymes were tested in included…

    • 1456 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Enzyme Catalysis Lab

    • 1096 Words
    • 5 Pages

    Enzyme catalysis was observed in order to analyze how changes in temperature, pH, enzyme concentration, and substrate concentration affected an enzyme-catalyzed reaction. This experiment analyzed the rate of enzyme-catalyzed reactions and observed the correlation between catalase activity and products formed. It was found out that the rate of an enzyme-catalyzed reaction starts off rapidly, decreases, and levels off or completely stops, and can be further affected by environmental factors, which play a crucial role in regulating enzymes and metabolic processes.…

    • 1096 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    Enzyme Lab

    • 338 Words
    • 10 Pages

    In our everyday lives, enzymes are used in our bodies, and in nature around us, to speed up the chemical reactions happening constantly, which happens by lowering the amount of activation energy needed to start various reactions. The way this works is by attaching the particular substrate to the active site of the enzyme, where it will start to aid the chemical reaction. Then, the allosteric site involves itself in forming the final 3D shape. For each specific reaction is a specific enzyme that helps speed up the reaction, and the reason for the variations of the enzymes is their unique protein structures. However, this means that once the structure of the enzyme is denatured and changed, the functions will most probably modify as well. In nature, this happens when the temperature and concentrations of different components are altered. In this lab experiment, we will be doing an in-depth research of exactly what happens to the enzymes, when it happens, and why it denatures the way it does.…

    • 338 Words
    • 10 Pages
    Satisfactory Essays
  • Better Essays

    Enzyme Formal Lab

    • 2542 Words
    • 11 Pages

    The purpose of this investigation is to figure out how temperature, pH, and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to occur, allowing vital processes such as digestion, ATP production, and DNA replication to occur efficiently. Using a Vernier Gas Pressure Sensor the rate of reaction of the enzyme catalase as it decomposed Hydrogen Peroxide in a test tube was measured and then results were put on the screen of a Macintosh computer using Logger Pro software and Vernier computer software. Several trials were then done with one factor, either temperature, pH, or enzyme concentration being altered, while the remaining two stayed constant. The rate of reaction of catalase should increase with enzyme concentration until a certain point until it reaches its limit. For temperature the rate of reaction of catalase should increase until the enzyme catalase denatures, and then after the rate should start to decrease. When exposed to different pH levels the rate of reaction of catalase should increase as the enzyme catalase reaches its optimal pH level, once it’s at that point the rate of reaction should then decrease as the pH level goes farther away from catalase’s optimal pH level. The data for the trials of pH and enzyme concentration support the expected hypotheses of how the reaction rate would be affected, however the data did not support the hypothesis made for the effects of temperature on an enzyme’s reaction rate due to divergent data from the expected results. (need this line or is conclusion and results of data and trends summarized with data supported hypotheses) ask if titel too long,,…

    • 2542 Words
    • 11 Pages
    Better Essays

Related Topics