and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the
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ABSTRACT Enzymes are highly specific and can distinguish isomers of the same molecule. The enzyme invertase specifically catalyzes the reaction of the conversion of sucrose to its individual carbohydrates glucose and fructose. It does not catalyse the reaction of maltose to 2 glucose or lactose to galactose. In this experiment‚ titrimetric and spectrophotometric methods were used to determine the specificity of invertase by determining the amount of glucose converted from the given disaccharides
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EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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EXTRACTION OF INVERTASE FROM YEAST AND EFFECT OF pH ON ENZYMATIC ACTIVITY Jenelle C. Faustino‚ John Gambit B. Garcia‚ Fatima S. Jusay‚ Oliver Alexander B. Lao and Eunice L. Licudine Group 4 2 E Medical Technology Biochemistry Laboratory ABSTRACT Enzymes are substances that are produced by living organisms and act as catalysts in order to speed up or chance a chemical reaction without changing itself at the end of the reaction. Invertase was extracted first from baker’s yeast. Determination
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Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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Caselet 1 Kinetic Engineering Limited (KEL) has created a buzz in biking circles with its announcement of introducing the 250 cc cruiser Aquila of Hyosung Motors of Korea. It has generated curiosity and interest among bike enthusiasts in the country. The bike is being promoted as a ’real’ cruiser. It is fitted with a 250 cc. v-twin‚ oil-cooled engine with four valves per cylinder. It has an output of 26 bhp. with top speed of over 130 kmph. The company is the first to introduce this high-end bike
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A KINETIC STUDY OF AN IODINE CLOCK REACTION PURPOSE To investigate the kinetics of the reaction that occurs between iodide and persulfate ion. You will: (1) determine the rate law‚ (2) determine the numerical value of the rate constant at room temperature‚ (3) explore the effect of temperature on the reaction and determine the activation energy (Ea)‚ and (4) investigate catalytic activity of selected metal ions on the reaction. INTRODUCTION Reaction times vary from picoseconds (10-12 seconds)
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Tro’s Chemistry Chapter 13 – Chemical Kinetics Page 1 of 13 Acknowledgements: Many of the images are adopted from Tro’s textbook‚ the only purpose of which is to enhance student learning. Key terms‚ concepts‚ skills: Refer to pp 599 – 601. Review questions: 3 – 24. Suggested problems: 25‚ 27‚ 33‚ 39‚ 43‚ 53‚ 57‚ 59‚ 69‚ 73‚ 75‚ 81‚ 93‚ 103. 13.1 & 2 Introduction to the Rate of a Chemical Reaction • kinetics is the study of the factors that affect the speed of a reaction and the mechanism by
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----------------------------------------------------- Abstract The interactions of Mg2+ and Zn2+ ions in the activation of non-specific tissue alkaline phosphatase were investigated using crude extracts of rat kidney. Activation of alkaline phosphatase by the metal ions was accompanied by changes in the kinetic parameters of pnitrophenylphosphate hydrolysis. The results suggest some synergistic interactions between Mg2+ and Zn2+ ions in promoting the hydrolysis of p-nitrophenylphosphate by alkaline
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