used to measure the concentration of protein in a solution [1]. There are several reagents that can be used to determine the concentration of protein in solution which are Bradford reagent‚ Biuret reagent‚ Lowry protein assay and many more. In this experiment‚ the Bradford Reagent is used to measure the protein concentration. Marion M. Bradford had developed the Bradford protein assay. The Bradford assay is a colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine
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Experiment AM1.4--Bending moments in a simply supported beam Student name JunJie Liu Student ID 1512042 Experiment Date 24 Nov 2014 Lab group Mech 7 Introduction In this lab report we show the basic methods of measuring bending moment at the “cut” assuming only simply supported beam with point loads (showed in figure 1) and illustrate the relationship among bending moment and distance between
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Tma05‚ Introduction to a de100 project on the likeness of a logo A fictitious educational experiment was created and conducted by a team on the DE100 module and therefore they had to maintain plan and undertake a project to pinpoint their potential findings; this therefore meant launching a logo for internet TV channel in order to address whether or not evaluative conditioning works in either experimental or control conditions. The logo was used to question if it would attract an audience and
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Review Sheet Exercise 12 Serological Testing NAME Kali Rothfuss LAB DATE/TIME 6/22/11 Positive and Negative Controls 1. Why are there a number of washing steps in serological tests? The are a number of steps needed in order to remove any non specific binding that may have occurred. 2. Describe how you would know that you had a “false positive” result. What does this mean for the rest of your results? A positive result with a negative control indicates a “false positive”
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------------------------------------------------- Griffith’s experiment Griffith’s experiment discovering the "transforming principle" in pneumococcus bacteria. Griffith’s experiment‚ reported in 1928 by Frederick Griffith‚[1] was one of the first experiments suggesting that bacteria are capable of transferring genetic information through a process known as transformation.[2][3] Griffith used two strains of pneumococcus (Streptococcus pneumoniae) bacteria which infect mice – a type III-S (smooth)
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transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated on 0.8% agarose gel at 40V for 8 h. De-purination (0.25N HCl) was done in glass pot for 15 min with shaking at room temperature. Subsequently rinse the gel in autoclaved distill
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agent‚ to positive serum‚ containing antibodies against that infecting agent‚ is called A) complement fixation. B) RIA. C) ELISA. D) seroconversion. 4. The serology test that may show the antigen-antibody complex as yellow-green under the microscope while using an ultraviolet light is known as the A) fluorescent antibody test. C) AIDS test. B) ELISA test. D) radioimmunoassay. 5. Immunological tests may determine the presence of A) antigen or antibody. C) only antigen. B) neither
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Abstract The aim of my research was to study automatic processes by replicating the previously carried out Stroop effect. The participants‚ 20 Richmond College students (10 boys and 10 girls) chosen by an opportunistic sample were taken into a quiet room separately‚ were presented with 6 lists of words‚ out of which 3 were congruent and the other 3 incongruent and the time taken for each participant to name the colour that the words were written in was measured and recorded. From
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Audit Proposal Do Private Laboratories provide HIV/AIDS Counselling and Testing? Nigeria as a country has a population of 167 million people (1) while her HIV prevalence rate is 3.4 %; an approximately 3.4million people are living with HIV/AIDS. This number represents the second highest HIV burden in the world second only to South Africa (2). The Government of Nigeria has intervened and put in place different measures to reduce this incidence to its barest minimum amongst which is the National
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Paper Towel Lab Report AP Biology 15 September 2011 Which Towel is The Quicker-Picker Upper! To determine which of the most popular paper towel brands such as Bounty and Marcal would be the Quicker-Picker-Upper I conducted the following experiment during which I dipped 6in X 6in squares of paper towels into 100mL and measured the amount of water absorbed by the towels in 20 seconds. After averaging all 3 trials for the paper towel brands that I tested I arrived to the conclusion that the
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