The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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Activity 1.1.5 ELISA 1. Explain why the antibodies allow scientists to target and identify specific disease agents. The antibodies are specific to the antigen they destroy. 2. Why is the secondary antibody used in the ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate? The enzyme catalyzes the oxidation of the substrate and turns the solution blue. 3. Disease samples from two patients are collected and subjected to serial dilutions before
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Activity 1.1.5: ELISA Introduction Given Sue’s diagnosis‚ all of the patients from the past two days need to be called back in for immediate testing. School officials are concerned about a possible outbreak of bacterial meningitis on campus. In order to diagnose bacterial meningitis‚ it is necessary to obtain a sample of cerebral spinal fluid using a spinal tap. Since this procedure is extremely invasive and painful‚ only those patients doctors feel are at greatest risk for the disease will
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unethical experiments on human subjects; regardless of what their condition is or if they have been diagnosed with a disease. Although‚ the use of humans for experiments to further develop cures for diseases‚ or to improve weaponry in war has always been a questionable practice. Hundreds of medical experiments have been performed by various doctors such as: The Tuskegee Syphilis Study by the U.S Public Health Service‚ the experiments conducted in Nazi Germany by Josef Mengele‚ the experiments performed
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PLANT GROWTH EXPERIMENT Numerous environmental variables influence plant growth. Three students each planted a seedling of the same genetic variety in the same type of container with equal amounts of soil from the same source. Their goal was to maximize their seedling’s growth by manipulating environmental conditions. Their data are shown below. Plant Seedling Mass (grams) Day 1 Day 30
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Evaluation Evaluation Experiment one: This experiment was quite accurate‚ as when it is compared to the manufactures line which is on the same graph‚ we can see that this line is at most only 0.4? different form the manufactures line. This is a percentage difference of approximately 8%‚ using the formula: Difference ? original X 100 This shows that the results were good‚ as 8% is a very small margin of error. The error bars on the graph show that the most inaccurate result was the 60cm result
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Nonverbal Experiments Everyone will do Experiment #1 – A Mute Point Choose 3 additional experiments – follow the directions and have fun! 1. A mute point – We will all do this on the same day! You can not talk for one entire day. Document your frustrations‚ your “oops” moments‚ reactions from others‚ and if any new revelations came about from not speaking. Make sure you wear your sign! 2. Disregarding the Norm Go to a crowded public space. Intentionally go against the grain in some
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self-regulation is emerged. So‚ where does it all begin? Childhood. Pre-school is a great place to start teaching self-regulation. An experiment that I believe to be a useful demonstration of educating children on their self-regulation is group games that promote leadership skills. Materials Needed for Experiment: • Pilot’s hats Procedure To begin this experiment the marshmallow test must be administered to the kids and the results must be recorded. We then separate the kids into two different
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Ballistic pendulum designed experiment Aim: to investigate how the mass of the projectile affects distance on the motion of a wooden block it strikes Hypothesis: the heavier the projectile the further the distance will be covered by the wooden block Variables: variables Identification Manipulation Independent Mass of projectile The four different masses will be used in different combinations Dependent distance Measured using tape measure Controlled 1. Height of retort stand 2. Release angle
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Surface Tension Steven Brignol Dr. Payne September 15 Introduction: Water’s ability to stick to itself is surface tension. In this lab we were able to measure and detect surface tension by dropping water‚ drop by drop‚ onto a penny. The quantity of droplets that fit on the penny was impressive. Hypothesis: The detergent will thicken the water making a tighter surface tension. Prediction: If soap increased the surface tension‚ then expansion will happen. Materials: (1) Pipette (2) Water
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