PCR: 1 μL of Herculase II Fusion DNA polymerase‚ 10 μL of 5X Herculase II reaction buffer‚ 0.5 μL of a 100mM solution of dNTPs (25 mM each)‚ 2 μL of a 10 μM solution of each primer‚ 100 ng of each upstream and downstream fragments‚ 200 ng of pyrG marker fragment and adjust to 50 μL of double-distilled water. 35. Lysis Buffer: to prepare 50 mL of buffer dissolve 23.6 g of Guanidine thiocyanate (118.16 g/L) in 25 mL of double-distilled water. Once dissolved add: 2.5 mL of 1 M Tris-HCl pH 7.0 (121.14
Premium DNA Molecular biology Protein
The Effect of Temperature on The Viscosity of Glycerol Research Question: What is the effect of increasing the independent variable of the temperature of glycerol (30ºC‚ 40ºC‚ 50ºC‚ 60ºC and 70ºC) (+ 0.5ºC) using a hotplate and measured using a thermometer (+ 0.05º)‚ on the dependent variable of time (secs + 0.2) taken for 50mL (+ 0.05ml) of glycerol to flow through a 50cm3 burette (+ 0.05cm3) into a 250 mL beaker (+ 12.5 mL) measured by a digital stopwatch (+ 0.2secs)‚ therefore indicating the
Premium Temperature Heat Viscosity
Introduction In a Grignard reaction‚ a Grignard reagent (R–MgX) adds to the carbonyl group in an aldehyde or ketone to form an alcohol (Figure 1). The reaction of a Grignard reagent with formaldehyde can be to synthesize a primary alcohol‚ with any other aldehyde can be used to synthesize a secondary alcohol‚ while the reaction with ketone is useful in the synthesis of a tertiary alcohol. Figure 1. General reaction mechanism of a Grignard Reaction The preparation of the Grignard reagent involves
Premium Acetic acid Acid Chemistry
Introduction DNA‚ or deoxyribonucleic acid‚ is a double stranded helical structure used to store genetic information in cellular organisms. DNA usually consists of two strands made up of nucleotides‚ each with a backbone of repeating units of phosphate groups and the sugar‚ deoxyribose‚ bonded by phosphodiester linkages. Since the deoxyribose has a specific orientation‚ DNA molecules have directionality so that DNA sequences are read 5’ to 3’. The 5’ end of DNA is characterized by the deoxyribose
Premium DNA Gene Genetics
The purpose of the lab was to find out the chemical elements based on the color of the flame that elements make by their reaction. The work we completed in the lab was testing the chemical effects of the flame from the chemicals. We tested different elements and wrote down our observations. I accomplished the knowledge of testing chemicals and making responses based on our observations.Electrons are the particles found in the chemicals that may be responsible for the production of colored light.
Premium Chemistry Fundamental physics concepts Chemical compound
Title: Studying SN1 and SN2 Reactions: Nucleophilic Substitutions at the saturated carbon Lab Partner: Jeszie Geronimo Objective and purpose: the purpose of this experiment is to convert a primary alcohol to an alkyl bromide using a Sn2 Reaction. Investigate some factors that influence the rate of Sn1 reactions. The second part of this lab will focus more on how unlike factors influence the rate of reactions in anSN1 reactions. The factors that we will be inspecting are the leaving groups‚ Cl-
Premium Nucleophile
Introduction – Lab Report What is a Chemical Reaction? A chemical reaction is a change in matter that produces one or more new substances. A chemical change or reaction occurs when bonds are broken and new ones are formed. The formation and dissolution of these bonds are dependent upon environment changes. Even without the usage of microscopes‚ chemical reactions are usually apparent to the naked eye. The two main kinds of changes that one can observe are the formation of new substances and changes
Premium Chemistry Chemical reaction Temperature
three reactions that occurred in the test tubes where you combined potassium iodide‚ KI‚ and hydrogen peroxide‚ H2O2 (in part 1) of the experiment? (Give a detailed explanation of any observations that you made‚ i.e.‚ what made the colour change‚ what reactions happened?) ANSWER: 2. Use the information below to develop the necessary calculations for the rate of reaction from the solutions in part B of the experiment • Calculate the initial molarity
Premium Chemistry Experiment Chemical reaction
The purpose of this experiment was to synthesize triphenylmethanol from a Grignard reagent. The Grignard reaction technique was used in this synthesis but due to the fact that it is such a strong nucleophile and base‚ it was important to prevent water from interfering with the Grignard reaction. Purity of the product was determined by measuring the melting point. Reagent Table: Structure Name Molecular formula Molar mass Density Melting point Boiling Point Diethyl ether C4H10O
Premium Magnesium Oxygen Diethyl ether
Organic Chemistry II Lab Diels Alder Reaction Purpose: In this experiment a Diels-Alder reaction was used to form the products. Cyclopentadiene and maleic anhydride were reacted together to form cis-Norbornene-5‚6-endo-dicarboxylic anhydride. 7-oxabicyclo{2.2.1}hept-5-ene-2‚3-dicarboxylic anhydride was also produced through a Diels-Alder reaction with the combination of furan and maleic anhydride. Equation: Procedure: Part 1 In a flask equipped with
Premium Chemistry Hydrogen Chemical reaction