2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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AGAROSE GEL ELECTROPHORESIS Gel electrophoresis is a widely used technique for the analysis of nucleic acids and proteins. Most every molecular biology research laboratory routinely uses agarose gel electrophoresis for the preparation and analysis of DNA. We will be using agarose gel electrophoresis to determine the presence and size of PCR products. Background: Electrophoresis is a method of separating substances based on the rate of movement while under the influence of an electric field
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DNA as a Key Witness Criminals‚ often unknowingly‚ leave parts of themselves behind. These pieces are not always visible to the untrained eye. Hair‚ skin‚ blood‚ and fingerprints all contain elements that are unique to each person. It is with DNA testing and fingerprinting‚ that criminals can be identified and crimes can be linked. This system of testing and matching has become the “most essential and reliable method of catching criminals” in the United States (Lynch 67). Advancing technology
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DNA fingerprinting is a way of identifying a specific individual‚ rather than simply identifying a species or some particular trait. It is also known as genetic fingerprinting or DNA profiling. As a technology‚ it has been around since at least 1985‚ when it was announced by its inventor‚ Sir Alec Jeffreys. DNA fingerprinting is currently used both for identifying paternity or maternity and for identifying criminals or victims. There is discussion of using DNA fingerprinting as a sort of personal
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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to that person‚ therefore it is a great resource for police to help locate people involved in a case. Families share many of the same traits in their DNA but people are unsure of whether or not they have similarities‚ In this experiment that question will be answered. There are three main fingerprint types: arches‚loops‚and whorls. (GeneEd - DNA Fingerprints 2003‚ April 12). The police take fingerprint samples from the crime scene or from involved people). Once the fingerprints are taken and
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Ligation of Lambda DNA pre-digested with EcoRI and HinDIII. Restriction of Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme used‚ determined
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Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page 7 to page 10)
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extraction and amplification of DNA (Mullis et. al. 1986). 2. PCR has the ability to isolate specific DNA sequences with the use of primers. This is done by denaturing the DNA (at 95o C) so it is able to anneal to the primers that specify a fragment to be amplified (Mullis et. at. 1986). These primes anneal to a specific sequence of DNA in order to amplify this desired sequence. Once the annealing process is complete‚ the primers are extended (at 30o C) by a DNA polymerase added to the reaction
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2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively charged‚ the gel is
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