BCH 369L–Biochemistry Laboratory Fall 2016 Module C Orlando Martinez uteid: olm298 Introduction The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin
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scene by comparing with the DNA samples. Polymerase Chain Reaction(PCR) is used to amplify the small amount of Deoxyribonucleic Acid (DNA) for forensic or genetic studies‚ which require necessary product and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using an electric current applied to the gel matrix‚ which causes the DNA samples to move towards
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homologous DNA sequences. It refers to a difference between samples of homologous DNA molecules that come from differing locations of restriction enzyme sites‚ and to a related laboratory technique by which these segments can be illustrated. In RFLP analysis‚ the DNA sample is broken into pieces (digested) by restriction enzymes and the resulting restriction fragments are separated according to their lengths by gel electrophoresis. Although now largely obsolete due to the rise of inexpensive DNA sequencing
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“copy”= DNA replication -when DNA is copied- interphase‚ S phase of cell cycle -recognition of origin site on DNA -concept of unwinding enzyme (helicase) -RNA primer (RNA primase) -DNA polymerase- adds complementary nucleotides to DNA template strand -concept of complementary relationship among bases-semiconservative antiparallel backbones and 5’ -> 3’ generation of new segments -DNA ligase- hooks Okazawki fragments together -Other- telomere replication‚ proofing by DNA polymerase
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thinking 1. DNA or deoxyribonucleic acid refers to the molecules that carry our genetic information. DNA can be found in blood‚ skin cells‚ tissues‚ muscles‚ bones‚ teeth‚ hair‚ and saliva. 2. Mitochondrial DNA is DNA that can be inherited from one’s mother and is found outside of the cell nucleus. 3. CODIS is a software program that contains the DNA profiles of convicted offenders‚ missing persons‚ crime scene evidence‚ and other sources. CODIS works by attempting to match the samples of DNA based on
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Ashley Crane Central Maine Community College Jurassic Park was a very popular film that entertained the notion that scientist could bring back an extinct species with DNA from mosquitos that were carefully preserved in amber. Now‚ a group of scientists are trying to bring back the great passenger pigeon‚ Ectopistes migratorious. According to the Firefly Encyclopedia of Birds‚ there were 3‚000 million passenger pigeons in the late 18th Century‚ making it one of the most abundant species in the world
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future is determined by their DNA. I wouldn’t want to live in this world‚ because your future and personal like/personality‚ you‚ are already chosen‚ so you have no choice of want you want your future to be like. In the movie GATTACA‚ Vincent has a dream to go to space but he can’t because of his DNA sequence. So he decides to become someone else in order to get a good job and fulfill his dream. Jerome has perfect DNA but he is disabled‚ so he agrees to give his DNA (skin cells‚ hair‚ nail cells
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can be either DNA or RNA. There is comparatively little genetic material for example E.coli genome is 1200 micrometres while a single human chromosome is between 12000 and 73000 micrometers in size. Unlike in eukaryotes the genetic material in viral prokaryotes can be single or double stranded RNA or DNA. It can also be linear or circular. Only in viruses can the genetic material be RNA. For example in HIV the virus can infect a host cell and use an enzyme transciptase to sequence a DNA molecule which
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Promoter is a site where‚ a. DNA polymerase binds b. Repressor binds c. RNA polymerase binds d. Inducer binds 3. _______________ enzyme forms RNA based on the information carried on DNA strand. a. DNA polymerase b. RNA polymerase c. DNA helicase d. A & C 4. Operator is a site where‚ a. DNA polymerase binds b. Repressor protein binds c. RNA polymerase binds d. DNA helicase binds 5. Mutation is defined as _________________. a. A permanent change in DNA b. A permanent change in RNA
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Exam 1: The complete set of hereditary information carried by an organism is its genome Human DNA contains 20% C on a molar basis. What are the mole percents of A‚ G‚ and T? A- 30% G- 20% T- 30% In a polynucleotide‚ a phosphate group is linked to the 3’ and 5’ carbons 2 pentoses Which of the following is a transition mutation? A-T —> G-C - transition= purine —> purine or pyrimidine —> pyrimidine - Purines- GA - Pyrimidines- CT The basic building block of nucleic acids is the nucleotide
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