Atrazine (1-chloro-3-ethylamino-5-isopropylamino-2‚ 4‚ 6-triazine‚ CAS: 1912-24-9) was purchased from Dr. Ehrenstorfer GmbH (97.7%‚ Germany). Ethyl acetate (99.8%‚ ANPEL Scientific Instrument (Shanghai) Co.‚ Ltd.) was used as solvent for standard solutions. Acetonitrile used as HPLC eluent was purchased from ANPEL Scientific Instrument (Shanghai) Co.‚ Ltd.‚ KH2PO4·12H2O‚ K2HPO4·2H2O‚ dichloromethane and other chemical reagents used in cultivated media were purchased from Sinopharm Chemical Reagent
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Liposomes of specific composition were prepared using a standard protocol. Briefly‚ Overnight hydration of lipid thin film obtained by high pressure vacuum evaporation of lipid mixture was done using Buffer A (Hepes-10 mM‚ KCL-150 mM‚ and 2mM EDTA‚ pH 7.4). This was followed by short periods of shaking and bath sonication for better retrieval of the lipids. Vesicles were generated from this initial mixture by freeze-thawing (10 cycles) and bath sonication for 20 min. 100 nm liposomes were produced
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Lab Report Macrocyclic Synthesis (Heme Analog) I. Purpose of Experiment and Introduction The purpose of this lab experiment is to prepare meso-tetraphenylporphin (TPP) and its copper‚ cobalt or zinc complex by condensing benzaldehyde with pyrrole in boiling propanoic acid. II. Introduction and Background There are two types of proteins that function as oxygen carriers: myoglobin (stores the oxygen) and hemoglobin (present in red blood cells and is responsible for oxygen transport)
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The carcinogenic nature of N-nitrosamines depends on the organs. For example‚ their targets for cancer in rodents include thyroid‚ pancreas‚ urinary bladder‚ trachea‚ lung‚ oral and nasal cavities‚ esophagus‚ kidney and liver. With respect to N-nitrosamides‚ skin‚ bone‚ peripheral nervous system‚ brain‚ small intestine and glandular stomach are targeted organs for cancers. Besides cancer‚ acute myelocytic leukemia and T and B cell lymphoma have also been triggered by N-nitrosamides. In table 8.1
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A. The scientific principle behind step A is all to do with burning the seaweed and turning into ash. To do this it had to be done with a Bunsen burner which was on the blue flame so it was at its hottest to turn the seaweed into ash. B. In step B the scientific principle is the filtration of the purified water. The reason for the ash being boiled was to extract the iodine ions. D. The scientific principles behind step D was the adding of hydrogen peroxide solution to the remaining
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Experiment 4: Heating Under Reflux- Synthesis of Salicylic Acid from Wintergreen Oil Precious Ahmed ID# 6463614 Section 07‚ Friday afternoon Lab experiment performed on: Friday‚ January 19‚ 2013 Lab report submitted on: Friday‚ January 24‚ 2013 Introduction Before the synthetic aspirin of today‚ salicylic acid‚ which is the important ingredient found in aspirin‚ was extracted naturally from methyl salicylate found in Wintergreen oil‚ which could be found in certain plants. The purpose
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Extraction and Evaporation: Separating the Components of "Panacetin" Submitted- July 17‚ 2012 Introduction The purpose of this lab is to investigate the composition of a compound suspected to be Panacetin‚ a type of pain-killer. Panacetin is typically made up of sucrose‚ aspirin‚ and acetaminophen‚ but the third component in this experiment is unknown. The unknown component is suspected to be a chemical relative of acetaminophen‚ either acetanilide or phenacetin. Using techniques such as
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Lab Report Enzymes (must be typed) Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL What is the Hypothesis of this experiment? (3 points) -The highest temperature will affect the reaction rate of enzymes. What is the dependent variable? (1 point) -Change of rate/product/color/absorbency. What is the independent variable? (1 pt) -The temperature. Explain in detail the procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times)
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Experimental set-up Observations Figure 1 Figure 2 Figure 3 Figure 1 show the crystallisation reaction when the saturated sodium chloride solution was added to the cool reaction mixture. The salt of p-Toluenesulphonic acid started forming. Figure 2 show the wet crystal after the precipitated salt was being filtered by suction. The wet crystal was light purple
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Experiment 11 Calorimetry and Hess’s Law Purpose- To determine the change in enthalpy for four reactions using calorimetry and Hess’s Law Procedures: A. Calibration of the Calorimeter 1. Obtain two copper cylinders and a Styrofoam cup with lid from your lab instructor. Check out a digital thermometer display from the storeroom window. 2. Set up a hot water bath using a 600mL beaker‚ ring stand‚ and Bunsen burner. Weigh the two copper cylinders
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