Microbiology report Gram stain Objectives: The aim of this experiment was to be able to stain bacteria correctly and be able to see it under the light microscope. In 1883 Hans Christian Gram discovered an important staining method that is used extensively today. The stain is called the Gram Stain. This experiment was done in order to differentiate microbes into two basic groups: Gram positive microbes and Gram negative microbes. The purpose of this experiment was to learn the gram staining method and to
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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4. Procedures 5-6 5. Results 7 6. Discussion8-9 7. Conclusion 10 8. Acknowledgments 11 9. Bibliography 12 10. Appendix 13 11. Forms 14 Introduction The type of bacteria found in milk can help society in many ways. For example‚ if testing goat’s milk‚ sheep’s milk‚ skim milk‚ and two percent milk‚ and the results are similar‚ and then the basic structure of the milks DNA is similar. This conclusion can come in use when products are being tested on cows‚ sheep‚ and
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testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe‚ meaning ATP can
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colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test | Purpose | Reagents | Observations | Results | Gram stain | To determine the Gram reaction of the bacterium | Crystal violet‚ Iodine‚ Alcohol‚ Sarasin | Pink rod | Gram-negative rods
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bacteria? Gram Stain This separation is based on differences in what physical trait? The structure of the cell walls. What is the iodine used for in this staining technique? Crystal violet is first used to stain the sample and iodine is used to affix it. Why is it important to heat fix the bacteria prior to the staining procedure? This procedure is done to kill any bacteria that may be on the slide already and this will insure a clean slide. If you had a bacteria sample that you knew to be gram positive
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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Question 1 of 12 2.0 Points All of the following are considered microbes except A.viruses B.bacteria C.protozoa Correct D.worms Answer Key: D Question 2 of 12 2.0 Points Microbiologists investigate problems by designing and carrying out experiments. What is true about the scientific method? A.a hypothesis is the definitive explanation to account for the observation and therefore does not need to be tested B.a prediction is the factor that can change
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