CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11‚ 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5 3013
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Separation Of A Dye Mixture Using Chromatography ********* AP Chemistry (3rd Block) Fauquier High School 8/ 31/ 13 ABSTRACT: The Rf values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents‚ H2O‚ Isopropyl alcohol‚ and a 2% NaCl solution‚ were introduced to the dotted chromatography strips to show three different ratios for each dye on
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Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
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useful solvent for many chemical processes. The solid was already in powder form therefore we did not have to crush it but it the unknown was in a tablet form‚ one would need to reduce it to a powdery-like form in order to perform this Thin-Layer Chromatography experiment. The powder solution was thoroughly mixed with a glass rod in the test tube containing
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Palladio‚ the Column‚ and the Utilisation of Columnar Forms from Late Imperial Roman Architecture Palladio‚ the Column‚ and the Utilisation of Columnar Forms from Late Imperial Roman Architecture Palladio revered the column. It was his favourite structural element and architectural ornament. Palladio also revered antiquity. He thoroughly documented the five orders in the first book of Quattro Libri‚ and devoted the fourth book to “the ancient temples” where columns are presented in their original
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Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
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I. Column Oriented Database A column-oriented DBMS is a database management system (DBMS) that stores data tables as sections of columns of data rather than as rows of data. In comparison‚ most relational DBMSs store data in rows. This column-oriented DBMS has advantages for data warehouses‚ customer relationship management (CRM) systems‚ and library card catalogs‚ and other ad hoc inquiry systems where aggregates are computed over large numbers of similar data items. It is possible to achieve
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Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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tested for by a possible employer include Cocaine (crack)‚ Amphetamines (crystal)‚ Opiates (codeine‚ morphine‚ heroin)‚ PCP (phencyclidine)‚ and Marijuana. Gas chromatography/mass spectrometry is used to test hair and urine samples of possible drug abusers or job applicants‚ and it is the best method for the testing of drug use. Gas chromatography and mass spectrometry are two different methods for identifying chemical substances‚ and the two instruments have be coupled together to perform a highly complementary
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Encyclopædia Britannica Paper chromatography is used to separate "dissolved chemical substances". An example of dissolved chemical substances could be the pigments that make up a specific color‚ for example in a marker. When a chromatography paper experiment is done on a black marker‚ the experiment tends to separate all the different pigments that make up the color. In this experiment‚ the substance investigated was a black water soluble Giotto marker pen. Paper chromatography has two phases‚ the
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