lab‚ caproic acid was synthesized in a multi-step process that involved the synthesis of three intermediates – diethyl n-butylmalonate‚ potassium n-butylmalonate‚ and n-butyl malonic acid respectively. An IR was used to characterize the starting material‚ n-bromobutane‚ and the first intermediate‚ diethyl n-butylmalonate; while IR and NMR were used to characterize the final product‚ caproic acid. Reactions‚ Mechanism and Theory Caproic acid a.k.a n-hexanoic acid is a carboxylic acid derived from
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EXPERIMENT 2 ACID DISSOCIATION CONSTANT OF AN INDICATOR DYE OBJECTIVES Using spectrophotometric method: determine the wavelengths at which the acid and base forms of the dye in aqueous medium exhibit maximum absorption; determine the molar absorptivities of the acid and base forms of the dye and estimate an unknown concentration of the dye in solution using the Beer-Lambert’s Law; and determine the acid dissociation constant of the indicator dye. THEORY The absorption or reflection of
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The acid-base titration curves help to find the pKa‚ Ka‚ and pH at equivalent point. At the beginning pH for HCl is 1.90 which is lower than the 3.28 for acetic acid; thus‚ strong acid (HCl) means lower pH and weak acid (acetic acid) means higher pH. Then at the equivalent point for the titration HCl-NaOH the pH is 7‚ which mans that is neutral‚ in other words there are enough NaOH mmol to neutralize the HCl mmol present; also‚ the solution contains only water and NaCl the salt derived from the titration
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Trimyrisitin to Sodium Myristate to Myrisitic Acid Mandy Boyle Chem 213‚ Section 002 Due Date: December 3‚ 2009 I. Introduction People encounter esters everyday in both natural and synthesized forms. Esters are present in a variety of common compounds‚ from fragrances to animal fat (McMurry‚ 2008). Although these esters can undergo many different important reactions‚ this lab is particularly interested in the hydrolysis of esters into carboxylic acids and alcohols. Companies such as Dove‚ Palmolive
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This paper is an analysis conducted on Nucleic Acids through a variety of tests specifically‚ Dische‚ Murexide‚ Wheeler-Johnson and Phosphate Tests in order to exemplify structural features of nucleic acids as well as identify the principle involved in each chemical test. Different procedures and different test compounds were applied‚ and results were noted as for changes in colors of precipitates or solutions. For Dische Test‚ light blue was obtained for RNA and dark violet for DNA. For Murexide
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Extraction of R’-NH2 For both of the organic acid and organic base extraction‚ the addition of the respective inorganic acid or inorganic base (HCl or NaOH) to the three-component mixture caused an immiscible appearance of the solution in the separatory funnel. By briskly shaking the separatory funnel with the mixed components created a build up of pressure which was released through the stopcock forming small disappearing bubbles within the tip of the separatory funnel. Once the funnel was placed
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Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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Abstract: Introduction: Materials: * Chemicals: Buffer solution‚ pH 7.0‚ 50 mL Phenolphthalein indicator solution‚ 1.0 %‚ 1 mL Potassium hydrogen phthalate‚ KHC8H4O4‚ 2 g sodium hydroxide solution‚ NaOH‚ 0.1 M‚ 150 mL Unknown weak acid‚ 1.5g Water‚ distilled or deionized * Equipment: Balance Stir bar Beaker‚ 250mL Oven Buret‚ 50 mL pH sensor Desiccator Rising stand and buret clamp Erlenmeyer flask‚ 125mL Wash bottle with distilled water Funnel Weighing dishes‚ 2
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TEST OF CONTROLS – REVENUE TRANSACTIONS |Audit Objective |Audit procedure |Findings | |General | | | |Validity |Observe
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Table 1. Observations from week 2 for the detection of ammonia using the Nessler’s reagent and from week 1 for the pH using bromothymol blue indicator with the inoculation of P. vulgaris‚ P. fluorescens‚ and B. Cereus in peptone broth. Tubes were incubated at room temperature for 7 days and 14 days. Soil Microorganism Nessler’s Reagent (color reaction pH (bromothymol blue) Our results pH (bromothymol blue) Class results P. vulgaris Deep yellow ++ 8.0 8.0‚ 7.5‚ 6-7‚ 11.5 P. fluorescens
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