electrophoresis can also be used as a method to separate and purify proteins. MATERIALS AND REAGENTS: 1. Electrophoresis tank with voltage power supply. 2. 2.5 x 12 cm sized cellulose acetate paper. 3. Paper to line the table. 4. Tray for the dye and acetic acid. 5. Tray for the buffer. 6. Forceps. 7. Capillary tubes. 8. Clock. 9. Filter paper. 10. Scissors. 11. Spectrophotometer (570 nm wavelength) 12. 5% (v/v) of acetic acid (glacial acetic acid 50 ml/L) 13. 0.2% of Ponceau S that
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process heating a liquid to form vapor which is then collected when cooled and separated from the original liquid. The purpose of this experiment was to determine the identity of an unknown substance using simple and fractional distillation. Gas chromatography analysis the components of a mixture by separation of the materials by their different boiling points. EXPERIMENTAL The simple distillation apparatus was assembled using a 10-mL round bottom flask‚ distillation head‚ thermometer‚ adapter‚ thermometer
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Ion Exchange Chromatography Discussion: The first exercise preformed in this lab was ion exchange chromatography. The purpose of this experiment is to separate molecules based on their differences in charge. Since it is based on charge the amino acids in the cation exchange column‚ if negatively charged‚ flow through the column first because they don’t want to bind to the sodium ions. The positively charged ions will elute last at the highest ph because they bind to the negatively charged beads
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Description of Scientific Interests Environmental Technology and Energy Technology‚ the two strongly connected‚ and very much socially relevant interdisciplinary fields have inspired me a lot right from my school days. My bachelors and masters in the broad fields of Biotechnology & Biochemical Engineering and Biotechnology & Bio-reaction Engineering‚ respectively‚ have prepared me well enough for pursuing further in the fields of Environmental Technology and Energy Technology. I have been very
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EXPERIMENT 4 ANALYSIS OF HYDROCARBON IN COMMON FUELS BY SOLID-PHASE MICROEXTRACTION (SPME) AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) Abstract In the analysis the solid-phase microextraction (SPME) and capillary gas chromatography/mass spectrometry (GC/MS) was developed for the identification of volatile compounds (hydrocarbon) in fuel. The samples was used is (kerosene‚ diesel‚ thinner and petrol) and one unknown. After the analyte was extracted by SPME in 20min‚ it directly injected
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Outline: Title The title of the lab should be at the top of the lab report. Background Information and Research 1. Give a simple explanation‚ in your own words‚ of what paper chromatography is and what it is used for. 2. Give at least three real-world uses for paper chromatography used in the fields of chemistry and biology. 3. Be sure to include references for any research conducted for this section. Purpose In one or two complete sentences‚ state the purpose of this laboratory
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Pre-Lab Extraction and Filtration 790 1-1 Mixture Separation 792 1-2 Water Purification 794 3-1 Conservation of Mass 798 4-1 Flame Tests 801 Pre-Lab Gravimetric Analysis 828 13-1 Separation of Pen Inks by Paper Chromatography 830 13-2 Colorimetry and Molarity 834 14-1 Testing Water 838 804 7-1 Separation of Salts by Fractional Crystallization 806 7-2 Naming Ionic Compounds 810 7-3 Determining the Empirical Formula of Magnesium Oxide
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Analysis of A. fischeri chDNA restriction digestion by Sal I enzyme after isolation and purification via agarose gel electrophoresis Bio 219-064 (Techniques in Molecular Biology) Group 5: Tung Nguyen‚ Uyen Tran‚ Amber Beckley‚ Danielle Exler Department of Biology‚ Drexel University‚ Philadelphia‚ Pennsylvania 19104 Submitted October 27‚ 2014 ________________________________________________________________________ Abstract Bioluminescence is one of the benefits that a deep sea organism received
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geochemistry has played an important role in many areas of exploration and production for fossil fuels. Many of the more recent developments can be seen to have developed in parallel with developments in analytical chemistry such as gas chromatography and gas chromatography−mass spectrometry. For the past two decades such analytical techniques have been used to search for trace amounts of compounds known as biomarkers present in oils and source rock extracts which can be used to provide valuable information
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NOTES AND COMMENT 343 DETERMINATION OF CHLOROPHYLL AND PHEO-PIGMENTS : SPECTROPHOTOMETRIC EQUATIONS~ It has been shown that chlorophyll degradation products may at times constitute a significant fraction of the total green pigments present in seawater (Yentsch and Menzel 1963; Lorenzen 1965; Yentsch 1965). These degraded forms‚ or inactive chlorophyll‚ absorb light in the red part of the spectrum; if they are present in concentrations significant relative to chlorophyll a‚ a serious error
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