Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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Viruses & Bacteria Title: Kirby-Bauer Method of Antibiotic Effectiveness Purpose: The purpose of this lab was to determine the effectiveness of certain antibiotics and determine their zone of inhibition Data: Name of Antibiotic Zone of inhibition (mm) Effectiveness Streptomycin 15mm Sensitive Chloramphenicol 29mm Sensitive Novobiocin 20mm Intermediate Neomycin 13.5mm Intermediate Tetracycline 22mm Sensitive Analysis: 1. Which antibiotic was the most effective? The most
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called gram stain is used in the identification of bacteria. Bacteria can be gram-positive‚ gram-negative‚ or gram-variable. Most bacteria however‚ are usually gram-positive or gram-negative. Gram-positive and gram-negative bacteria react differently to staining because of the differences in their cell walls. Bacterial cell walls contain a polymer called peptidoglycan. Gram-positive bacteria have more of this polymer than gram-negative bacteria. They are also less complex than gram-negative cell
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Amber McMillian May 26‚ 2009 Bacterial Vaginosis This is a vagina condition also referred to as Gardnerella Vaginitis that can produce vaginal discharge that is a result from an overgrowth of normal bacteria. This will cause a woman to have a vaginal discharge with a foul odor. It is not dangerous but it can cause bad symptoms. If any woman has this problem they should also be checked for more serious infections such as Chlamydia and Gonorrhea. The normal symptoms are excessive vagina discharge
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shapes as before‚ just with sharper outlines. Questions: A. What are the advantages of using bleach as a disinfectant? The disadvantages? The advantages of using 70% alcohol? The disadvantages? 70% alcohol mixtures are capable of killing most bacteria within five minutes. However‚ the mixture is ineffective against spores and has limited effect on viruses. Additionally‚ the 70%
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Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial colony was isolated form Mycorrhizal spores‚ and further
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Gut Bacteria In today’s world‚ many people have a very negative connotation when they hear the word “bacteria”. Bacteria have been the cause to many diseases‚ however bacteria do have some benefits and uses. There are thousands and thousands of different types of bacteria in the world and one group of bacteria‚ Gut Flora‚ has become evident in the bacterial community for being beneficial. Gut Flora is a group of bacteria that consists of microorganisms that live in the digestive tracts of animals
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is researching a newly-discovered species of bacteria. At time t = 0 hours‚ he puts one hundred bacteria into what he has determined to be a favorable growth medium. Six hours later‚ he measures 450 bacteria. a. Assuming exponential growth‚ what is the growth constant "k" for the bacteria? (Round k to two decimal places.) b. After 10 hours‚ how many bacteria will there be? c. When will there be 10‚000 bacteria? 10. A certain type of bacteria‚ given a favorable growth medium‚ doubles in
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Introduction Although bacteria is microscopic in size‚ it is largely important in the healthcare field‚ environmental work‚ food preparation‚ as well as many other industries. In particular‚ it is essential that healthcare workers be able to identify the species of bacteria invading a human reservoir in order to prescribe the correct antibiotic that will kill that species. For the purpose of bacteria identification‚ numerous tests have been devised to find out the exact species in question. However
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ANALYSIS OF BACTERIA GROWTH Abstract Bacteria can be found everywhere on the earth. Bacteria are single-celled organisms that lack a nucleus‚ mitochondria‚ and chloroplasts and are surrounded by a cell wall containing a peptidoglycan layer. They are defined by their small size and wide range of shapes. In this experiment we separated organisms in a mixed culture to study the colony morphology and physical characteristics and determine which organism was present in higher numbers‚ as determined
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