Enzyme Study Questions 1. Definitions/terminology: o enzyme: a protein molecule that catalyzes chemical reactions without itself being destroyed or altered o catalyst: a substance that increases the rate of a chemical reaction but is not consumed or changed by it. o substrate: a substance upon which the enzyme acts. o denaturation: the partial or total alteration of the structure of a protein without change in covalent structure by the action of certain
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One of the most terrifying and frightening words in the English language is cancer. Many children and adults of all ages can be diagnosed with a rare type of bone cancer each year. The harmful effects of this disease cannot only be detrimental but deadly too. Thus‚ with early detection‚ diagnosis‚ and proper treatment‚ individuals have a better chance of survival when treated early. First‚ bone cancer is a deadly disease that consists of malignant tumor that forms in the bone. It can also destroy
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Calcinosis cutis is rare condition signified by calcium deposition in skin and subcutaneous tissues. In all cases of calcinosis cutis‚ insoluble compounds of calcium are deposited within the skin due to local and/or systemic factors. These calcium salts consist primarily of hydroxyapatite crystals or amorphous calcium phosphate. It is commonly seen in blacks‚ and no apparent gender predilection is noted (6‚7) From the pathogenesis point of view‚ the condition is classified as metastatic‚ dystrophic
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Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate‚ as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore
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Capparis spinosa L. belongs to the family Capparaceae is distributed throughout India and commonly known as Maratimokku in Tamil. In the present study attempts were made to assess the phytochemical‚ nutraceutical and chemical composition‚ pharmacological properties and safety profiles of C. spinosa. Powder microscopy revealed characteristics features of C. spinosa. Preliminary phytochemical screening of flower buds of C. spinosa L. revealed the presence of alkaloids‚ reducing sugars‚ carbohydrates
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bacterial challenge have been implicated with the incidence and prevalence of caries. The delicate balance of demineralization and remineralization to which hard tissues are subjected to dwells on the level of salivary calcium‚ phosphorus and alkaline phosphatase. At the early stage of demineralization‚ salivary calcium and phosphorus diffuse into the subsurface causing a reversal. However in children with high caries risk‚ repeated or increased
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for children suffering from HPP. Asfotase alfa is an important advance for many patients with HPP‚ their families‚ and the medical community because it can effectively replace in the skeleton the deficient enzyme called tissue non-specific alkaline phosphatase‚” said Michael Whyte‚ M.D.‚“Without treatment‚ many newborns and infants with HPP fail to develop a normal rib cage and die from not being able to breathe‚ and young children with HPP can suffer from muscle weakness. Treatment with asfotase
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Basic Reagents and Techniques of Molecular Biology Laboratory Protocols Ausubel F‚ Brent R‚ Kingston R‚ Moore D‚ Seidman J‚ Smith J‚ Struhl K. Current Protocols in molecular biology. John Wiley & Sons‚ New York‚ 1996. Sambrook J‚ Fritsch EF‚ Maniatis T. Molecular Cloning: a Laboratory Manual‚ 2nd ed.‚ Cold Spring Harbor Laboratory‚ Cold Spring Harbor‚ N.Y.‚ 1989. Basic Tools for DNA Manipulation and Molecular Genetic Analysis Enzymes Vectors Gel Electrophoresis PCR Southern blotting
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DIGESTIVE ENZYMES AND THEIR FUNCTIONS Mouth -the oral cavity or the mouth contains salivary glands that secrete a wide range of enzymes to aid the first step of metabolism of food. The salivary gland secretes the enzyme lysozyme which has an antibacterial action. The enzymes secreted by the mouth mainly provide protection against bacteria. Betaine helps to maintain a fluid balance inside the mouth. The lists of digestive enzymes secreted by the oral cavity are mentioned in the table. Enzymes
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Features and outcomes of spontaneous bacterial peritonitis in Egypt‚ single center experience prospective study Spontaneous bacterial peritonitis (SBP) is the infection of the ascetic fluid that occur in the absence of a visceral perforation and in the absence of an intraabdominal inflammatory focus such as abscess‚ acute pancreatitis or cholecystitis (Guarner C and Soriano G‚ 2007). Since its initial description in 1964‚ research has transformed spontaneous bacterial peritonitis
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