Enzymes Introduction This study allows the investigation of enzymatic reactions behavior. An enzyme is a protein catalyst reaction by lowering the activation energy required for that reaction. The enzyme is unaltered at the completion of the reaction. In this stimulation the amount of product produced during the course of an enzymatic product produced during the course of an enzymatic reaction will be measured. Hypothesis 1: What is the estimate optimal of ph? Hypothesis 2: What is the estimate optimal of temperature
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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Biology EEI Effect of Inorganic and Organic Fertilisers on Yield and Growth of Tomatoes By Yash Teacher: Mrs. Elphick TABLE OF CONTENTS Abstract................................................................................3 Introduction..........................................................................4 Materials and Methods.........................................................6 Contents of fertilisers...........................................................6 Procedure....
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causing the body to not have enough acids in the urine. These factors are all very different‚ but are interconnected because an imbalance in any of these factors can lead to imbalances in other areas as well (Wise‚ 2010). The importance of this lab is to analyze factors that may affect urine concentration‚ pH‚ and rate of production (flow rate) though multiple scenarios over a period of 60 minutes. My hypothesis for the urine analysis experiment is that the urine volumes will increase because
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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DNA Extraction of a Musa acuminata Biology Lab Report \Analyze and Interpret A number of steps are required to isolate DNA from cellular contents. Describe what happens at each step‚ and why it acts to separate the parts of the cell. /6 There are three specific steps required to isolate DNA from its cellular contents. The steps used to remove and expose DNA from its cell are: breaking down the food type you are using by crushing it‚ for example a banana or strawberries‚ exposing the substance
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Radioisotopic labels would be used in experiments to identify semi-conservative replication in prokaryotes. Because we anticipated that a labeled DNA would have different density with unlabeled‚ which means‚ by analyzing the different density of DNAs‚ we can determine which of DNA is labeled‚ half-labeled or unlabeled. To this end‚ I will use c13 label the bacteria and abruptly change carbon source with C12. Then I will collect four samples in different time and analyze the results from centrifugal
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Investigating Effects of duration of heating on permeability of beetroot cell membrane Introduction: Cell membranes contain many different types of molecules which have different roles in the overall structure of the membrane. Phospholipids form a bilayer‚ which is the basic structure of the membrane. Their non-polar tails form a barrier to most water soluble substances. Membrane proteins serves as channels for transport of metabolites‚ some act as enzymes or carriers‚ while some are receptors
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wberry DNA Lab ReportMeredith Anderson Mrs. Berman Biology October 17‚ 2012 Extracting DNA from Strawberries Problem/Question: Successfully extract the DNA from strawberries. Theory/Hypothesis: N/A Variables: Independent – strawberries Dependant – the DNA Controlled – 450 ml of water‚ 50 ml of soap‚ 1 tsp of salt‚ 5 ml isopropyl alcohol Apparatus and Materials: N/A Procedure and Control of Variables: N/A Data Collected: See attached paper. Data Processing and Presentation:
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Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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