CHEM120 Week 7 iLab: DNA, mRNA, and Protein

CHEM120 Week 7 iLab: DNA, mRNA, and Protein (30 points)

Name: Kaylee Klefman

Complete the two questions below. Each question has four parts. This assignment is two pages long.

Question 1:

For the following double-stranded DNA sequence,
-CATTGACCGTAA-
-GTAACTGGCATT-

Answer the following questions:

a) Assume that RNA polymerase will read the top strand of DNA as the “template” to synthesize mRNA. What will be the sequence of the mRNA synthesized? (3 points)

The new mrna sequence will be GUAACUGGCAUU

b) Based upon the mRNA sequence above, what is the peptide sequence? (4 points)

The peptide swquence will be GUA-ACU-GGC-AUU

If there was a mutation in the DNA sequence above resulting in the following DNA sequence,
-GATTGACCGTAA-
-CTAACTGGCATT-

c) What would be the resulting mRNA, assuming RNA polymerase will use the DNA sequence, -GATTGACCGTAA- ? (4 points)

Thw new mena sequence will be CUAACUGGCAUU

d) Based upon the mRNA sequence above, what is the peptide sequence? (4 points)

The peptide sequence will be CUA-ACU-GGC-AUU which will translate to Leu-Thr-Gly-lle

Question 2:

For the following DNA sequence,
-CCCTTAGATGGA-
-GGGAATCTACCT-

Answer the following questions:

a) Assume that RNA polymerase will read the top strand of DNA as the “template” to synthesize mRNA. What will be the sequence of the mRNA synthesized? (3 points)

GGGAAUCUACCU

b) Based upon the mRNA sequence above, what is the peptide sequence? (4 points)

GGG-AAU-CUA-CCU which will translate to alanine-asparagine-leucine-proline

If there was a mutation in the DNA sequence above resulting in the following DNA sequence,
-CTCTTAGATGGA-
-GAGAATCTACCT-

c) What would be the resulting mRNA, assuming RNA polymerase will use the DNA sequence, - CTCTTAGATGGA - ? (4 points)
GAGAAUCUACCU

d) Based upon the mRNA sequence above, what is the peptide sequence? (4 points)
GAG-AAU-CUA-CCU which translates to glutamate-asparagine-leucine-proline