Ceratopteris Fern Growth and Analysis Essay Example
Ceratopteris Fern Growth and Analysis: Observing Germination and Effects of Ammonium Nitrate Treatment
I: Abstract
Observing the effects of ammonium nitrate has on the germination days of C-Fern gametophytes by constructing an experiment with two spore-sown petri dishes, one control and one treatment – a normal nutrient-rich agar petri dish and an ammonium nitrate-containing petri dish respectively. The two petri dishes were each inoculated with three drops of spore suspension by a pipet, then spores were spread by a sterilized-bent paper clip to allow even distribution of spores. Gametophyte germination occurred approximately two weeks after inoculation for the control, and gametophyte germination occurred approximately three weeks after inoculation for the treatment; thus possibly suggesting ammonium nitrate worked to hinder the mitotic cell division in the haploid gametophyte.
II: Introduction
C-Fern begins with a haploid spore known as the spore imbibition (Hickok, Warne 2009), then after 3 to 4 days the spore will develop into a gametophyte via mitosis after germination. This is a photoautotrophic process. The gametophyte will then undergo differentiation (Hickok, Warne 2009), to form the antheridium and the archegonium after 6 to 8 days (Hickok, Warne 2009). Following the gametophyte displaying its hermaphrodite form, sexual maturity allows cross-fertilization to occur between the sperm from antheridium and the egg from archegonium. After one sperm fertilizes the egg, a zygote forms, thus forming a diploid cell (Hoshizaki 2001). The formation of the zygote exemplifies sporophytes’ alternation of generations via the diploid cell completing mitosis and meiosis yet still retaining a full set of genetic material. In other words, alternation of generation refers to going from a multicellular diploid form to a multicellular haploid form. (Hickok, Warne 2009). The fertilized egg develops into a sporophyte by mitosis to form a
I: Abstract
Observing the effects of ammonium nitrate has on the germination days of C-Fern gametophytes by constructing an experiment with two spore-sown petri dishes, one control and one treatment – a normal nutrient-rich agar petri dish and an ammonium nitrate-containing petri dish respectively. The two petri dishes were each inoculated with three drops of spore suspension by a pipet, then spores were spread by a sterilized-bent paper clip to allow even distribution of spores. Gametophyte germination occurred approximately two weeks after inoculation for the control, and gametophyte germination occurred approximately three weeks after inoculation for the treatment; thus possibly suggesting ammonium nitrate worked to hinder the mitotic cell division in the haploid gametophyte.
II: Introduction
C-Fern begins with a haploid spore known as the spore imbibition (Hickok, Warne 2009), then after 3 to 4 days the spore will develop into a gametophyte via mitosis after germination. This is a photoautotrophic process. The gametophyte will then undergo differentiation (Hickok, Warne 2009), to form the antheridium and the archegonium after 6 to 8 days (Hickok, Warne 2009). Following the gametophyte displaying its hermaphrodite form, sexual maturity allows cross-fertilization to occur between the sperm from antheridium and the egg from archegonium. After one sperm fertilizes the egg, a zygote forms, thus forming a diploid cell (Hoshizaki 2001). The formation of the zygote exemplifies sporophytes’ alternation of generations via the diploid cell completing mitosis and meiosis yet still retaining a full set of genetic material. In other words, alternation of generation refers to going from a multicellular diploid form to a multicellular haploid form. (Hickok, Warne 2009). The fertilized egg develops into a sporophyte by mitosis to form a