Wala Pa
Kamias (Averrhoa bilimbi) LEAVES AND FRUIT EXTRACT: ITS ANTIBACTERIAL EFFECT ON Escherichia coli, Staphylococcus aureus AND Salmonella enteritidis
A Science Investigatory Project as
an Entry for the 2008 School Level Science Fair
Ellaine M. Andales Angelica Mae J. Flores Hannah C. Mendoza Research Proponents
Mrs. Noreen T. Catis Project Adviser
REGIONAL SCIENCE HIGH SCHOOL Malasiga, San Roque, Zamboanga City
October, 2008
ABSTRACT
This research study aims to prevent diseases caused by Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. The study makes use of kamias (Averrhoa bilimbi) leaves and fruit extract as an antibacterial agent that is more or equally effective than the commercialized one and at the same time, available in our environment. Ten Replicates on ten Petri dishes were prepared for each bacteria. Pure cultures of the three bacteria were spread on the replicates. After extracting the needed substances from the kamias leaves and fruit extract, the researchers were able to begin the experimentation. Six treatments were made in the set-up-Treatment A as 100% water served as the negative control; Treatment B as 100% Kamias leaves extract; Treatment C as 50% Distilled water and 50% Kamias leaves extract; Treatment D as 100% antibiotics was used as the positive control because of its known antibacterial properties;
A Science Investigatory Project as
an Entry for the 2008 School Level Science Fair
Ellaine M. Andales Angelica Mae J. Flores Hannah C. Mendoza Research Proponents
Mrs. Noreen T. Catis Project Adviser
REGIONAL SCIENCE HIGH SCHOOL Malasiga, San Roque, Zamboanga City
October, 2008
ABSTRACT
This research study aims to prevent diseases caused by Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. The study makes use of kamias (Averrhoa bilimbi) leaves and fruit extract as an antibacterial agent that is more or equally effective than the commercialized one and at the same time, available in our environment. Ten Replicates on ten Petri dishes were prepared for each bacteria. Pure cultures of the three bacteria were spread on the replicates. After extracting the needed substances from the kamias leaves and fruit extract, the researchers were able to begin the experimentation. Six treatments were made in the set-up-Treatment A as 100% water served as the negative control; Treatment B as 100% Kamias leaves extract; Treatment C as 50% Distilled water and 50% Kamias leaves extract; Treatment D as 100% antibiotics was used as the positive control because of its known antibacterial properties;