Chap4 thesis experiment
CHAPTER 4
RESULTS AND DISCUSSION
4.1. Angelika keiskei (Ashitaba )crude non-polar extract The crude non-polar extract from the plant Angelika keiskei (Ashitaba) was tested for the anti-bacterial property by using the Kirby – Bauer Disk Diffusion test. There were four 25uL of Ashitaba non-polar extract with different concentrations used which were 12.5 %, 25%, 50% and 100%. In line with this, respective positive controls of the two specimens and negative control were also used. The results are discussed below.
4.1.1. Test against gram positive organism
Triplicates of plates were used to identify the anti-bacterial property of Ashitaba non-polar extract against gram positive organism, Staphylococcus aureus. There were six disk namely the different concentrations of Ashitaba extract (12.5 %, 25%, 50%, 100%), the positive control ( Penicillin) and the negative control ( distilled water).
Table 4.1 Results of anti- bacterial property of Ashitaba extracts against Staphylococcus aureus sample Zone of inhibition (mm)
12.5 %
25 %
50 %
100 %
Distilled water
Penicillin
A
0 mm
0 mm
0 mm
0 mm
0 mm 8. 0mm
B
0 mm
0 mm
0 mm
0 mm
0 mm
7.5 mm
C
0 mm
0 mm
0 mm
0 mm
0 mm
8.0 mm
Table 4.2 Interpretation of the anti- bacterial property of Ashitaba extracts against Staphylococcus aureus sample Zone of inhibition
12.5 %
25 %
50 %
100 %
Distilled water
Penicillin
A Susceptible Susceptible
Susceptible
Susceptible Susceptible
Resistant
B
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant
C
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant
Base on the table 4.1 and 4.2, the different concentrations of Ashitaba extracts (12.5 %, 25%, 50 % and 100 %) didn’t show any zone of inhibition which concludes susceptibility to the positive organism, Staphylococcus aureus.
Based on http://bsac.org.uk/wp-content/uploads/2012/02/Table-10.pdf ( pa biblio na lang pls
RESULTS AND DISCUSSION
4.1. Angelika keiskei (Ashitaba )crude non-polar extract The crude non-polar extract from the plant Angelika keiskei (Ashitaba) was tested for the anti-bacterial property by using the Kirby – Bauer Disk Diffusion test. There were four 25uL of Ashitaba non-polar extract with different concentrations used which were 12.5 %, 25%, 50% and 100%. In line with this, respective positive controls of the two specimens and negative control were also used. The results are discussed below.
4.1.1. Test against gram positive organism
Triplicates of plates were used to identify the anti-bacterial property of Ashitaba non-polar extract against gram positive organism, Staphylococcus aureus. There were six disk namely the different concentrations of Ashitaba extract (12.5 %, 25%, 50%, 100%), the positive control ( Penicillin) and the negative control ( distilled water).
Table 4.1 Results of anti- bacterial property of Ashitaba extracts against Staphylococcus aureus sample Zone of inhibition (mm)
12.5 %
25 %
50 %
100 %
Distilled water
Penicillin
A
0 mm
0 mm
0 mm
0 mm
0 mm 8. 0mm
B
0 mm
0 mm
0 mm
0 mm
0 mm
7.5 mm
C
0 mm
0 mm
0 mm
0 mm
0 mm
8.0 mm
Table 4.2 Interpretation of the anti- bacterial property of Ashitaba extracts against Staphylococcus aureus sample Zone of inhibition
12.5 %
25 %
50 %
100 %
Distilled water
Penicillin
A Susceptible Susceptible
Susceptible
Susceptible Susceptible
Resistant
B
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant
C
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant
Base on the table 4.1 and 4.2, the different concentrations of Ashitaba extracts (12.5 %, 25%, 50 % and 100 %) didn’t show any zone of inhibition which concludes susceptibility to the positive organism, Staphylococcus aureus.
Based on http://bsac.org.uk/wp-content/uploads/2012/02/Table-10.pdf ( pa biblio na lang pls