Preview

Substrate Concentration Investigation

Satisfactory Essays
Open Document
Open Document
179 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Substrate Concentration Investigation
For a reaction to occur, you need a substrate (catechol). The reaction will occur faster if you have an enzyme. A reaction without an enzyme will still take place, but at a slower rate. If the substrate is mixed with a greater amount of distilled water, there is more enzymes needed in order for a reaction to occur; as the reaction is directly dependent on the enzymes. At low concentration of substrate, increasing the substrate concentration resulted in an increase rate. Above a certain level, the rate no longer changes when substrate concentration increased because the enzyme is saturated with substrate.
The rate of an enzyme-catalyzed reaction increases as the temperature is raised. If the temperature is too high, the enzyme will start to

You May Also Find These Documents Helpful

  • Better Essays

    Crayfish Lab Report

    • 2156 Words
    • 9 Pages

    metabolic processes are able to occur at a faster rate; thus, leading an increase in…

    • 2156 Words
    • 9 Pages
    Better Essays
  • Satisfactory Essays

    1. As shown in the graph above, the rate of activity increases as the enzyme concentration is increased. In summarization, this means that the more enzymes that are available, allow for more substrates to be converted into product within a smaller amount of time. However, the substrate becomes a limiting factor and when there are no more substrates to be broken down, the reaction rate becomes constant.…

    • 315 Words
    • 1 Page
    Satisfactory Essays
  • Satisfactory Essays

    Biog 1440 Week 3

    • 269 Words
    • 2 Pages

    A’ may be saturated and their reactions run at the same maximum velocity. However, at…

    • 269 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    If the temperature was increased, the rate of reaction slows. If the temperature of the HCl was raised by 10°C, the activity will increase by 50-100%. This did not happen with the results shown in the chart above. Even small temperature changes such as 1-2°C can change the effect of the enzymes by 10-20%.…

    • 468 Words
    • 2 Pages
    Good Essays
  • Good Essays

    AP Bio Lab Report

    • 814 Words
    • 8 Pages

    up the chemical reaction while low temperatures will slow it down. This is because temperature has a…

    • 814 Words
    • 8 Pages
    Good Essays
  • Good Essays

    Enzyme Lab Report

    • 1060 Words
    • 5 Pages

    The increasing temperature increases molecular motion and may increase the number of times an enzyme contacts and combines with a substrate molecule. Temperature may also influence the shape of the enzyme molecule, making it fit better with the substrate.…

    • 1060 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Therefore, we know that the rate and the extent of the reaction increases, (as measured by color change) due to greater enzyme activity.…

    • 616 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Lab report

    • 352 Words
    • 2 Pages

    Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease.…

    • 352 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Peroxidase Experiment

    • 1061 Words
    • 5 Pages

    In regards to peroxidase, several points can be made about the overall effects of these factors. Factors such as temperature and pH have been shown to have an effect on the performance of enzymes (Vishwanatha, K.S., Food Chemistry. 2: 402-407; 2009). Adjusting the temperature will produce one of two results; if the enzymatic reaction occurs and the enzyme is colder than the optimum temperature, fewer collisions between enzyme and substrate will occur thus reducing the rate of the reaction. On the other hand, if the enzyme has been heated to a higher temperature than the optimum but not high enough to denature the enzyme, more of these collisions will occur and the reaction rate should speed up (Table 2, Graph 2). The second of the common factors is pH. Everything in the world has extremes on each end of the spectrum where the body, cell, etc. stops reacting to its environment. This goes for enzymes as well. If a solution is too basic or too acidic in comparison to the enzymes optimum pH, the enzyme will begin to degrade until it eventually stops reacting all together. It is important that students and biologists understand these and other optimal conditions for enzymes to react in. For the pH aspect of the experiment the group predicted, through reading of the laboratory manual and general knowledge of enzyme properties, that the rate would vary based on both…

    • 1061 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    Bio lab

    • 511 Words
    • 2 Pages

    As the environment and temperature is changed from 0 °c to 20°c to 95°c, the absorbance of catechol and catecholase reaction will increase over time. The rate of reaction increases as the temperature increases.…

    • 511 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Enzyme Activity Lab Report

    • 1074 Words
    • 5 Pages

    In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance, which gave us a lower rate for the enzyme reaction to complete, and vice versa for higher.…

    • 1074 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    Enzyme Lab

    • 1094 Words
    • 5 Pages

    This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased, then the reaction rate would also increase. To test our question, we varied a combination of substrate and buffer, totaling 6mL, with a constant amount of 2 drops of catalyst. The enzyme catalyst, peroxidase, increased the rate of the reaction. The results of our experiment can be found by comparing the reaction rates for each trial. These rates are actually the slopes of the lines that were graphed during each reaction. The trend of data…

    • 1094 Words
    • 5 Pages
    Powerful Essays
  • Satisfactory Essays

    Enzyme Lab

    • 431 Words
    • 2 Pages

    2. What do you think will happen to the rate of reaction if the concentration of enzyme is…

    • 431 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    The reaction goes faster initially. The relationship is linear. As the substrate concentration increase the initial reaction goes faster.…

    • 470 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    When comparing the three different Substrate Concentrations (3%, 1% and 0.3%) as which one was the fastest to react, I hypothesized that the reaction would occur the fastest with the 3% hydrogen peroxide (the highest amount of concentration) because the higher the concentration the more faster the reaction occurs and helps produce or break up the enzyme. After testing all three of my substrate concentrations with 10 trials each, my data showed (first graph) that the 3% substrate concentration had the fastest average rate at 0.19 of distance and time occurring and my second data chart (table chart) showed an estimate time of 15-25 seconds. The second highest average rate of the reaction was at 0.08 of distance and time occurring and the estimated reaction time was between 25-50 seconds.…

    • 468 Words
    • 2 Pages
    Good Essays