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Research Paper Fusarium Wilt on Abaca

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Research Paper Fusarium Wilt on Abaca
Induction of Resistance in Abaca Against
Wilt Disease Complex caused by
Fusarium oxysporum f. sp. cubense (E. F. Smith) and
Ralstonia solanacearum (Smith) Yabuuchi et. al.
Using Chemical and Biological Elicitors

Abstract Resistance in plants has been reported to be inducible using biological and or chemical elicitors. Fusarium wilt and bacterial wilt diseases are two major diseases hampering abaca production in the Philippines. This study was conducted to evaluate the efficacy of biological and chemical eliciors of resistance to control wilt disease complex in abaca caused by Fusarium oxysporum f. sp. cubense (FOC) and Ralstonia solanacearum. Suspected biological elicitors (Fomes and Xanthomonas oryzae pv. oryzae or Xoo) and known chemical elicitors (Asprin or Acetyl Salicylic acid and Boost) were sprayed to the abaca tissue cultured seedlings at 15 days interval together with water and Nordox (fungicide with bactericidal activity) as checks. The sprayed seedlings were challenged by simultaneous inoculation with FOC and Ralstonia solanacearum. The parameters used to compare the treatments were: percent infected plants, disease severity rating, percent area of vascular discoloration and number of remaining live plants. Results show that Boost and Fomes resulted to an effective control of the wilt disease complex which were comparable or even better compared to Nordox, the fungicide check. Xoo and Acetyl Salicylic Acid on the other hand produced higher disease compared to the control. This study has demonstrated the potential of Fomes and Boost as biological and chemical elicitors of resistance, respectively in abaca which protected it from wilt disease complex.

Keywords: Abaca, SAR, chemical elicitors, biological elicitors, Fusarium wilt, Bacterial wilt

------------------------------------------------- 1 Student Researcher, VSU 2 Associate Professor, DPM, VSU and Research Adviser

INTRODUCTION Abaca



References: 1) BASTASA, G.N. and A.A. BALIAD. 2005. Biological control of fusarium wilt of abaca (Fusarium oxysporum) with Trichoderma and Yeast. http://cropscience Philippines.blogspot.com/2006/032005-vol30-no-2-v30n02p01-02.html 2) BORINES, L.M 3) BORINES, L.M. SALAMAT, E.E. CARDINES, R.B. 2007. Reaction of abaca (Musa textiles Nee) accessions and varieties to Fusarium wilt caused by Fusarium oxysporum f. sp. cubense. (E.F. Smith) Snyd. And Hanns. Annals of Tropical Research. Volume 29 (No.1). 4) EL-MOUGY, N.S., R.ABB-L-KAREEM and M.A.ABB-ALLA. 2002. Postharvest Diseases Control: Preventive Effect of Chitosan and Bioagents Against Green and Gray Moulds of Apple Fruits. Egypt. J.Phytopathol., 30: 99-113. 5) FIDA 6) GLEASON, M. 1999. Sick Plants take aspirin. http://www.extension.iastate.edu/newsrel/1999/jan99/jan9901.htm 7) GORLACH, J., S 8) HERANO, S., C. ITAKURA, H. SEINO, Y. AKIYAMA,I. NOTATA, N. KANBARA and N. KAWAKAMI. (1990). Chitosan as an Ingredient for Domestic Animal Feeds. J. Agric. Food Chem., 38:1214-1217. 9) KUTCHITSU, K., M. KIKUYAMA and N. SHIBUYA. 1993. N-acetylchito-oligosaccharides, Biotic Elicitor for Phytoalexin Production, Induced Transient Membrane Depolarization in Suspension-Cultured Rice Cells. Protoplasma. 174:79-81. 10) MALAMY, J., CARR, J.P., KLESSIG, D.F., and RASKIN, I. (1990). Salicylic Acid: A Likely Endogenous Signal in the Resistance Response of Tobacco to Viral Infection. Science 250,1002-1004. 11) METRAUX, J.-P., SIGNER, H., RYALS, J., WARD, E., WYSS-BENZ, M., GAUDIN. J., RASCHDORF, K., SCHMID, E., BLUM, W.,and INVERARDI, B. (1990). Increase in Salicylic Acid at the Onset of Systemic Acquired Resistance in Cucumber. Science 250, 1004-1006. 14) UKNES, S., WINTER, A., DELANEY, T., VERNOOIJ, B., MORSE, A., FRIEDRICH, L., NYE, G., POTTER, S., WARD, E., and RYALS, J. (1993). Biological Induction of Systemic Acquired Resistance in Arabidopsis. Mol. Plant-Microbe Interact. 6,692-698.

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