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Article Summary of Vicia faba experiment

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Article Summary of Vicia faba experiment
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Ameirs SM, Farah OR. 1976. Cytological effects of pesticides VIII. Effects of the carbamate pesticides “IPC”, “Rogor”, and “Duphar”, on Vicia faba. Cytologia. 41, 3-4: (597-606).

Topic—Pesticide Effects, Meiosis, Yield

Context
This study investigate the effects of common pesticides, “Rogor”, “Duphar”, and “IPC” (o-isopropyl-N-phenyl carbamate), on Vicia faba, also known as the broad bean.

The objectives of this study were to determine the effects of three different pesticides on 1) meiosis, 2) pollen viability, 3) and the yield and root-mitosis of Vicia faba plants for three generations.

Methods critical to this study were: 1) soaking seeds in the three different pesticides, 2) spray treatments of three different pesticides, 3) permanent root-tip squash preparations stained after Feulgen technique.

Major results from this research include:
1. “Spraying plants at the flowering stage with “IPC” affected the highest percentage of abnormal PMCs” (Ameirs and Farah 1976).
2. Pesticide did not affect pollen viability
3. Seeds that were soaked in “Rogor” and “Duphar” showed a decrease in the average number of pods and weight of seeds/plants. This occurred in the 1st generation only.
4. Spraying plants with “Rogor” or “IPC” showed in increase in the average number of pods/plant only in the 2nd generation. This increase decreased in the 3rd generation
5. “The transmission of chromosomal aberrations to the following generations is very low after both seed-soak and spray-treatments” (Ameirs and Farah 1976).
6. All three pesticides have no harmful effect on the yield.

Synopsis
Spraying plants with “IPC” caused a high percentage of abnormal PMCs. “IPC” also caused tetraploid PMCs. Pesticides used in this study had no harmful effect on the yield. If an increase or decrease in yield occurred, it returned to normal in the following generation.

Relevance: This study discusses the effects of pesticides on Vicia faba which relates to our

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