Protein Delivery Through Oral Route
A REPORT ON Protein/ Peptide Drug delivery through Oral route
Submitted in fulfillment of the Study in Advanced Topic (BITS G513)
By S. Lakshmi Anusha (2008HS08605H)
BIRLA INSTITUTE OF TECHNOLOGY & SCIENCE, PILANI HYDERABAD CAMPUS 29th April, 2013.
1
A REPORT ON Protein/ Peptide Drug delivery through Oral route
Submitted in fulfillment of the Study in Advanced Topic (BITS G513) By S. Lakshmi Anusha (2008HS08605H)
UNDER THE SUPERVISION OF Dr. Punna Rao Ravi Assistant Professor, Department of Pharmacy.
BIRLA INSTITUTE OF TECHNOLOGY & SCIENCE, PILANI HYDERABAD CAMPUS 29th April, 2013.
2
Acknowledgement
I owe thanks to many people who have helped and supported me during the writing of this report.
I express my sincere gratitude to Dr.Punna Rao Ravi, the guide of the project, for guiding and correcting my work with patience, attention and care. I am grateful for the moral support and guidance given by him in different matters regarding the topic. I would like to express my deep gratitude to BITS, Pilani for providing me this opportunity.
The guidance and support received from my family and well-wishers, was vital for the writing of this project report. I am grateful for their constant support and help.
3
Table of Contents
1. 2. Introduction……………………………………………………………………….………6 Physicochemical properties of proteins………………………………………….………..6 2.1. 2.2. 2.3 2.4 2.5 3. Molecular weight Solubility Hydrophobicity Structure pH solubility
Instabilities of proteins……………………………………………………………………8 3.1 3.2 Physical Instabilities Chemical Instabilities
4.
Physiological Barriers to oral protein drug delivery……………………………………..9 4.1 Physical barrier 4.1.1. Paracellular pathway 4.1.2 Transcellular pathway Biochemical barrier
4.2. 5.
Formulation strategies………………………………………………………………….14 5.1. 5.2. 5.3 5.4 5.5 5.6 5.7 5.8 Absorption enhancers Enzyme inhibitors Hydrogels Mucoadhesive systems Liposomes Nanoparticles Microparticles Cyclodextrins
Submitted in fulfillment of the Study in Advanced Topic (BITS G513)
By S. Lakshmi Anusha (2008HS08605H)
BIRLA INSTITUTE OF TECHNOLOGY & SCIENCE, PILANI HYDERABAD CAMPUS 29th April, 2013.
1
A REPORT ON Protein/ Peptide Drug delivery through Oral route
Submitted in fulfillment of the Study in Advanced Topic (BITS G513) By S. Lakshmi Anusha (2008HS08605H)
UNDER THE SUPERVISION OF Dr. Punna Rao Ravi Assistant Professor, Department of Pharmacy.
BIRLA INSTITUTE OF TECHNOLOGY & SCIENCE, PILANI HYDERABAD CAMPUS 29th April, 2013.
2
Acknowledgement
I owe thanks to many people who have helped and supported me during the writing of this report.
I express my sincere gratitude to Dr.Punna Rao Ravi, the guide of the project, for guiding and correcting my work with patience, attention and care. I am grateful for the moral support and guidance given by him in different matters regarding the topic. I would like to express my deep gratitude to BITS, Pilani for providing me this opportunity.
The guidance and support received from my family and well-wishers, was vital for the writing of this project report. I am grateful for their constant support and help.
3
Table of Contents
1. 2. Introduction……………………………………………………………………….………6 Physicochemical properties of proteins………………………………………….………..6 2.1. 2.2. 2.3 2.4 2.5 3. Molecular weight Solubility Hydrophobicity Structure pH solubility
Instabilities of proteins……………………………………………………………………8 3.1 3.2 Physical Instabilities Chemical Instabilities
4.
Physiological Barriers to oral protein drug delivery……………………………………..9 4.1 Physical barrier 4.1.1. Paracellular pathway 4.1.2 Transcellular pathway Biochemical barrier
4.2. 5.
Formulation strategies………………………………………………………………….14 5.1. 5.2. 5.3 5.4 5.5 5.6 5.7 5.8 Absorption enhancers Enzyme inhibitors Hydrogels Mucoadhesive systems Liposomes Nanoparticles Microparticles Cyclodextrins