Franco lab report enzyme
LABORATORY REPORT
Activity:
Enzyme Activity
Name:
Daniel Franco
Instructor:
Professor Jennifer Frere
Date:
03.08.2015
Predictions
Sucrase will have the greatest activity at pH 6
Sucrase will have the greatest activity at
60 °C (140 °F)
Sucrase activity decreases with increasing sucrose concentration
Materials and Methods
Effect of pH on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable pH Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable temperature Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable amount of substrate (sucrose) present
Controlled Variables temperature, pH, sucrase + sucrose incubation time
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity.
Optimal density is measured because it measures the amount of glucose and fructose produced.
5. Explain why denatured sucrase was used as a control.
Denatured sucrose was used as the control because activated sucrose was used to increase the activity.
Laboratory Report/ Daniel Franco/ Enzyme Activity/ Professor Jennifer Frere/ 03.08.2015/ Page [1] of [4]
6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration.
In order to decrease enzyme activity.
Results
Table 1: Effect of pH on Sucrase Activity
1
2
3
average
ph 3
0.173
0.166
0.148
0.162
ph 4
0.329
0.310
0.322
0.320
Optical Density ph 6
0.945
0.954
0.959
0.953
ph 5
0.672
0.652
0.641
0.655
ph 7
0.807
0.768
0.818
0.798
ph 8
Activity:
Enzyme Activity
Name:
Daniel Franco
Instructor:
Professor Jennifer Frere
Date:
03.08.2015
Predictions
Sucrase will have the greatest activity at pH 6
Sucrase will have the greatest activity at
60 °C (140 °F)
Sucrase activity decreases with increasing sucrose concentration
Materials and Methods
Effect of pH on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable pH Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable temperature Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable amount of substrate (sucrose) present
Controlled Variables temperature, pH, sucrase + sucrose incubation time
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity.
Optimal density is measured because it measures the amount of glucose and fructose produced.
5. Explain why denatured sucrase was used as a control.
Denatured sucrose was used as the control because activated sucrose was used to increase the activity.
Laboratory Report/ Daniel Franco/ Enzyme Activity/ Professor Jennifer Frere/ 03.08.2015/ Page [1] of [4]
6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration.
In order to decrease enzyme activity.
Results
Table 1: Effect of pH on Sucrase Activity
1
2
3
average
ph 3
0.173
0.166
0.148
0.162
ph 4
0.329
0.310
0.322
0.320
Optical Density ph 6
0.945
0.954
0.959
0.953
ph 5
0.672
0.652
0.641
0.655
ph 7
0.807
0.768
0.818
0.798
ph 8