enzyme report
In this lab we used a solution of lactase to test the chemical and physiological properties of this particular enzyme and determined whether the lactase came from human cells or bacterial cells. In the statistical analysis statistical formulas and techniques are used to analyze the significance of a set of data and the validity of the conclusions made based on that data. These are some terms and definitions that will be crucial to understanding the validity of this experiment. An enzyme is a protein that acts as a catalyst to lower the activation energy required for reactions to progress in the cells. Null hypothesis states that there will be no difference between the result of two separate variables A and B. The null hypothesis states in regards to the enzyme experiment that lactase will not bind preferentially, or more specifically, to maltose or lactose. Before a null hypothesis can be rejected we must notice a large difference between glucose produced from maltose versus lactose. Alternate Hypothesis states the opposite of null in that there will be differences between the results of A and B. Probability is an indication of likelihood very similar to a percent chance. All probabilities are between 0 and 1, with probability zero indicating an event is impossible and one indicating an event is certain to occur. T-value or t-Test is what is used to determine whether or not the null hypothesis is valid. This t-value can be used to create a p value which in turn will determine whether the results are statistically significant or not. Based on what I know about the effect of temperature on the enzymatic activity of lactase, I hypothesize that higher temperatures will cause denaturing in the enzyme causing it to be less effective and lower temperatures will more conducive to enzymatic activity, and a higher pH will be more conducive to enzymatic activity.
For the materials and methods refer to the online lab manual.
The statistics for the experiments are as
For the materials and methods refer to the online lab manual.
The statistics for the experiments are as