Catechol Oxidase Lab Report
Catechol oxidase is found in animals and plants. Catechol oxidase is the enzyme that catalyzes the production of Ortho- quinone. This reaction produces Ortho- quinone, water, and browning of a potato when exposed to air. The purpose of this experiment was to observe the behavior of reaction rate when enzyme or substrate concentration is changed. The enzyme in this experiment was catechol oxidase, extracted from a potato. In the experiment, when substrate concentration was held constant, and the different enzyme serial dilutions were being tested, the reaction rate was expected reaction rate was expected to increase until all enzymes are saturated, then the reaction rate would level off. First, dilute the enzyme and substrate. After,