Catalase Lab
Abstract In this laboratory exercise we studied enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.
Each lab was assigned to a group A, B, C and D in our class performed this experiment. The data presented in this report will reflect the average rates of change in O2 ml/min of our class results. In the later discussion sections, it will become …show more content…
This group loaded the reaction chamber with 4 extract soaked disks. They used 3% H2O2 with a pH of 3 and then ran experiment they recorded their data in Table 3.
d. Then they repeated the experiment using 3% H2O2 with a pH of 5 and recorded the results in Table 3
e. Then they repeated the experiment using 3% H2O2 with a pH of 7 and recorded the results in Table 3
f. Then they repeated the experiment using 3% H2O2 with a pH of 9 and recorded the results in Table 3
g. Then they repeated the experiment using 3% H2O2 with a pH of 11 and recorded the results in Table 3
3. Cleaned up and Washed their hands
4. Recorded and calculated the rate of reaction per minute (see Table 3)
5. Created a line graph to show their data (see Graph 3)
6. Analyzed their data and created a written overview of the results (see results for procedure C)
Group D procedure: The influence of Temperature on Catalase Activity
1. Prepared filter disks
a. Collected and mixed liver extract
b. They put 4 ml of the extract in a test tube and placed it in boiling water for five minutes. Then they put the boiled extract into a petri dish and soaked 4 disks in it
c. Then they pour the rest of the liver extract into a small petri
Each lab was assigned to a group A, B, C and D in our class performed this experiment. The data presented in this report will reflect the average rates of change in O2 ml/min of our class results. In the later discussion sections, it will become …show more content…
This group loaded the reaction chamber with 4 extract soaked disks. They used 3% H2O2 with a pH of 3 and then ran experiment they recorded their data in Table 3.
d. Then they repeated the experiment using 3% H2O2 with a pH of 5 and recorded the results in Table 3
e. Then they repeated the experiment using 3% H2O2 with a pH of 7 and recorded the results in Table 3
f. Then they repeated the experiment using 3% H2O2 with a pH of 9 and recorded the results in Table 3
g. Then they repeated the experiment using 3% H2O2 with a pH of 11 and recorded the results in Table 3
3. Cleaned up and Washed their hands
4. Recorded and calculated the rate of reaction per minute (see Table 3)
5. Created a line graph to show their data (see Graph 3)
6. Analyzed their data and created a written overview of the results (see results for procedure C)
Group D procedure: The influence of Temperature on Catalase Activity
1. Prepared filter disks
a. Collected and mixed liver extract
b. They put 4 ml of the extract in a test tube and placed it in boiling water for five minutes. Then they put the boiled extract into a petri dish and soaked 4 disks in it
c. Then they pour the rest of the liver extract into a small petri