Catechol Oxidase Lab Report
The Production of Benzoquinone from a variety of environmental conditions in potato juice extract
Introduction
The presence of quinones prevents infection of microorganisms because it is toxic to them (Danyk, H, 2013). Quinones are produced by the oxidation of phenolic compound of catechol. Enzymes are used to speed up chemical reactions in cells (Danyk, H, 2013). The enzyme catechol oxidase is used to speed up the production of benzoquinone which is to help in infection prevention. In this study catechol oxidase was combined with potato juice extract and water. To provide more results of the enzymes productivity patterns the solutions; temperatures were changed, alterations were made in pH, and the amount of concentration …show more content…
The extent of the denaturation of the bonded enzymes is affected by the emphasis of the pH change. In cases where the pH change is very minimal there is the possibility that the correct structure can become regenerated completely from its optimum pH becoming reached again. Ideally this procedure will result in substances that are more basic and acidic to produce less benzoquinone and substances that have a pH of seven, or close to seven, will produce a higher concentration of benzoquinone because of the preferred neutrality that a pH of seven has. When preforming this procedure one must begin with five test tubes that are occupied with the correct amount of substance that has the pH of 4, 6, 7, 8, and 10 which will be used. Once this buffer is created for each tube, 10 drops of potato juice as well as catechol oxidase containing the enzymes must be added and shaken in order to equally distribute all the components so that a possible change in pH can occur. Once placed in a 3 degree water bath the variety of colors in the substances due to the new pH change would have had a chance to completely set, making it possible to determine which pH is ideal for the most amount of benzoquinone to be …show more content…
Being at these temperatures are closest to room temperature, which is 25-degreesF, the enzyme produced best stored at this temperature.
Discussion
Production Of Benzoquinone With Graph Data
Through the group results, seen in figure1, figure 2, and figure 3, the mean brought very similar absorption readings. The catechol oxidase with potato juice extract seemed to vary in production in pH data collected, though there was still gradual increase in production. Temperature solutions showed common production the 70- degrees F temperature and 3-degrees F temperature. As for the number of drops of catechol oxidase added to the solution proved that more of the enzyme present will lead to greater production of benzoquinone.
Common Errors and Ways to Improve
The amounts of drops that were put into the test tubes may not have been exactly the correct amount. This was exemplified through the test tube labeled 70 degrees. The absorption value was 90, most likely because more drops of potato juice extract or catechol were added to the test tube then needed. These unexpected results could be tested by redoing the procedure and being critical about how many drops each test tube was given.
Production of
Introduction
The presence of quinones prevents infection of microorganisms because it is toxic to them (Danyk, H, 2013). Quinones are produced by the oxidation of phenolic compound of catechol. Enzymes are used to speed up chemical reactions in cells (Danyk, H, 2013). The enzyme catechol oxidase is used to speed up the production of benzoquinone which is to help in infection prevention. In this study catechol oxidase was combined with potato juice extract and water. To provide more results of the enzymes productivity patterns the solutions; temperatures were changed, alterations were made in pH, and the amount of concentration …show more content…
The extent of the denaturation of the bonded enzymes is affected by the emphasis of the pH change. In cases where the pH change is very minimal there is the possibility that the correct structure can become regenerated completely from its optimum pH becoming reached again. Ideally this procedure will result in substances that are more basic and acidic to produce less benzoquinone and substances that have a pH of seven, or close to seven, will produce a higher concentration of benzoquinone because of the preferred neutrality that a pH of seven has. When preforming this procedure one must begin with five test tubes that are occupied with the correct amount of substance that has the pH of 4, 6, 7, 8, and 10 which will be used. Once this buffer is created for each tube, 10 drops of potato juice as well as catechol oxidase containing the enzymes must be added and shaken in order to equally distribute all the components so that a possible change in pH can occur. Once placed in a 3 degree water bath the variety of colors in the substances due to the new pH change would have had a chance to completely set, making it possible to determine which pH is ideal for the most amount of benzoquinone to be …show more content…
Being at these temperatures are closest to room temperature, which is 25-degreesF, the enzyme produced best stored at this temperature.
Discussion
Production Of Benzoquinone With Graph Data
Through the group results, seen in figure1, figure 2, and figure 3, the mean brought very similar absorption readings. The catechol oxidase with potato juice extract seemed to vary in production in pH data collected, though there was still gradual increase in production. Temperature solutions showed common production the 70- degrees F temperature and 3-degrees F temperature. As for the number of drops of catechol oxidase added to the solution proved that more of the enzyme present will lead to greater production of benzoquinone.
Common Errors and Ways to Improve
The amounts of drops that were put into the test tubes may not have been exactly the correct amount. This was exemplified through the test tube labeled 70 degrees. The absorption value was 90, most likely because more drops of potato juice extract or catechol were added to the test tube then needed. These unexpected results could be tested by redoing the procedure and being critical about how many drops each test tube was given.
Production of