Antiseptic And Disinfectant Lab Report
Effects of Antiseptics and Disinfectants
Purpose: The purpose of this lab is to discover substances that can slow the growth of, or kill, microorganisms.
Hypothesis: The antiseptic that will prevent the growth of bacteria the most is iodine. The disinfectant that will prevent the growth of bacteria the most is bleach.
Procedure:
To begin the lab, turn the agar plate over and divide the plate into four quadrants and label the agar plate whether you used the E. coli or B. megaterium and number the quadrants 1 through 4. Please keep in mind that one pair will test the E. coli and Environment 1 or 2, and one pair will test B. megaterium and Environment 1 or 2. After, you will need to swab the E. coli and B. megaterium on two different nutrient agar plates using a sterile disposable inoculating loop. Remember not to dig in into the agar or the agar might break. Then, you will choose two environmental samples (rat, turtle or fountain) and do the same steps you used for the E. coli or B. megaterium. When you have swabbed …show more content…
First of all, in the agar plate that contained e. coli, the antiseptic, iodine, prevented most of the growth by 12 mm and the disinfectant, bleach, prevented most of the growth by 5 mm. In the B. megaterium agar plate, the antiseptic, rubbing alcohol, prevented most of the growth by 20 mm and the disinfectant, bleach, prevented most of the growth by 10 mm. In the Environment 1 (turtle) agar plate, the antiseptic, iodine, prevented most of the growth by 5 mm and the disinfectant, Lysol, prevented most of the growth by 8 mm. Lastly, in the Environment 2 (rat) agar plate, the antiseptic, rubbing alcohol, prevented most of the growth by 1 mm and the disinfectant, Lysol, prevented most of the growth by 6 mm. There were no mistakes or errors during the
Purpose: The purpose of this lab is to discover substances that can slow the growth of, or kill, microorganisms.
Hypothesis: The antiseptic that will prevent the growth of bacteria the most is iodine. The disinfectant that will prevent the growth of bacteria the most is bleach.
Procedure:
To begin the lab, turn the agar plate over and divide the plate into four quadrants and label the agar plate whether you used the E. coli or B. megaterium and number the quadrants 1 through 4. Please keep in mind that one pair will test the E. coli and Environment 1 or 2, and one pair will test B. megaterium and Environment 1 or 2. After, you will need to swab the E. coli and B. megaterium on two different nutrient agar plates using a sterile disposable inoculating loop. Remember not to dig in into the agar or the agar might break. Then, you will choose two environmental samples (rat, turtle or fountain) and do the same steps you used for the E. coli or B. megaterium. When you have swabbed …show more content…
First of all, in the agar plate that contained e. coli, the antiseptic, iodine, prevented most of the growth by 12 mm and the disinfectant, bleach, prevented most of the growth by 5 mm. In the B. megaterium agar plate, the antiseptic, rubbing alcohol, prevented most of the growth by 20 mm and the disinfectant, bleach, prevented most of the growth by 10 mm. In the Environment 1 (turtle) agar plate, the antiseptic, iodine, prevented most of the growth by 5 mm and the disinfectant, Lysol, prevented most of the growth by 8 mm. Lastly, in the Environment 2 (rat) agar plate, the antiseptic, rubbing alcohol, prevented most of the growth by 1 mm and the disinfectant, Lysol, prevented most of the growth by 6 mm. There were no mistakes or errors during the