PREVALENCE OF ESCHERICHIA COLI ON PHILIPPINE PESO BILL WITHIN THE VICINITY OF ILOILO CITY 2012-2013 A Research Paper Presented to The Faculty College of Medical Laboratory Science Department Central Philippine University Iloilo City In Partial Fulfillment of the Requirement for the Research 1 JUSTINE GRACE G. JIMENEA ISAAC JAMES T. LEDESMA MAJA HANNAH LOU L. LOJA ROBERTO R. MANALO JR. JIRAH L. PEDROSA MARCH 2013 CHAPTER 1 INTRODUCTION Background of the Study A classic
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Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
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chain of goods. In 1998‚ FedEx acquired Caliber and its subsidiaries. The acquisition gave FedEx a sure advantage that it is now capable to providing more transportation services to other segments upstream in the supply chain. Along with internet and e-business‚ FedEx seize the opportunity to collaborate with customers in providing full supply chain management system. However‚ in the midst of acquisition and maintaining competitive advantage on technologies‚ FedEx’s profits have not been as per expectation
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Escherichia coli and Staphylococcus albus Some bacteria’s require a certain amount of light in order to function properly and there fore survive. Escherichia coli is found in the gut and intestines of mammals. Staphylococcus albus is generally a skin dwelling bacteria Aim: To test whether the amount and type of light that the bacteria’s E.coli and S.albus are subject to causes changes in their growth rates and habits. To test whether the growth rates and habits of the bacteria’s E. coli and S
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Life of Escherichia Coli Escherichia Coli 0157:H7 bacterium can live in the intestines of cattle‚ therefore meat can become infected during slaughtering. You can contract this bacterium by eating undercooked meat‚ especially ground beef. Escherichia Coli 0157:H7 can also be contracted by eating contaminated fruits or vegetables‚ as well by drinking raw fruit juices or milk‚ or sewage contaminated water. Infection can also be spread via person to person. Escherichia Coli 0157:H7 can also be
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Transformation of E. coli with your unknown plasmid Learning Goals: Insert your uncut unknown plasmid into chemically competent DH-5 E.coli cells and use antibiotic resistance to confirm the success of the transformation. You should familiarize yourself with the various methods of transformation and the advantages/disadvantages of each type. You should also understand how heat shock transformation works and how chemically competent cells make this type of transformation possible. For this transformation
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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On The Transformation and Transformation Number of Special Graphs A Special Problem Presented to the Faculty of the Department of Mathematics and Statistics College of Arts and Sciences University of Southeastern Philippines In Partial Fulfillment of the Requirements for the Degree of Bachelor of Science in Mathematics Romelyn D. Villamor April 2009 Abstract This paper introduces a new operation on graphs called transformation. A transformation is applicable on simple connected
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GENETIC TRANSFORMATION Edited by María Alejandra Alvarez Genetic Transformation Edited by María Alejandra Alvarez Published by InTech Janeza Trdine 9‚ 51000 Rijeka‚ Croatia Copyright © 2011 InTech All chapters are Open Access articles distributed under the Creative Commons Non Commercial Share Alike Attribution 3.0 license‚ which permits to copy‚ distribute‚ transmit‚ and adapt the work in any medium‚ so long as the original work is properly cited. After this work has been published
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Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can
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