EXTRACTION AND CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase‚ 1.03 g egg white albumin‚ and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine
Premium Protein Amino acid Solubility
Research question/Aim Extract copper from copper oxide by reduction using butane gas Hypothesis The butane gas will reduce the copper oxide and leave behind pure copper. This will allow us to find the empirical formula of the copper oxide as well as the masses of oxygen and copper Variables Dependant variable The dependant variable will be the reduction of the copper oxide which will be calculated at different temperatures. Independent variable The independent variable here
Premium Mass Heat Oxygen
SOP: Solid phase extraction of cylindrospermopsin in filtered water samples Document identifier: SOP_TOXIC_UDU_05F Prepared by: James S. Metcalf and Geoffrey A. Codd‚ UDU Date: 7 July 2005 1 Introduction In order to determine the concentration of cylindrospermopsin in the extracellular fraction of filtered environmental waters‚ solid phase extraction (SPE) is necessary to concentrate the toxin to concentrations capable of being detected by HPLC. 2 Experimental 2.1 Materials
Premium High performance liquid chromatography Chromatography Glass
Berry DNA Extraction Lab I. Problem Statement: Successfully extracting the DNA from strawberries. II. Background info: DNA is the genetic information in organisms that codes for proteins‚ and controls the cells function. The DNA is a double helix molecule that is formed by various nucleotides which include Adenine‚ Thymine‚ Guanine‚ and Cytosine with sugar phosphate. Different organisms can have larger or smaller genes. DNA is also what makes up chromosomes during cell replication. Strawberries
Premium DNA
Extracting DNA from Wheat Germ Cells Criteria to be assessed CE Introduction: DNA is the abbreviation for deoxyribonucleic acid. DNA is found in the nucleus of every cell & it stores the information that makes up living organisms. It is a double helix of 4 nucleotides. It contains a code that allows the body to make up proteins. The nucleotides are Cytosine‚ Guanine‚ Adenine and Thymine. (1) Wheat germ comes from wheat seeds. The ’ germ ’ is the embryo‚ which is the part of
Premium DNA Gene
Separation of a Carboxylic Acid from a Neutral Compound by Extraction Reference: Smith‚ Chapter 2 (Acids and Bases) Introduction Carboxylic acids and phenols are two families of organic compounds that contain carbon‚ hydrogen and oxygen‚ and also react with water to yield an excess of hydronium ions over hydroxide ions. Pure water has a pH of 7‚ which means it has a hydronium ion concentration‚ [H3O+] of 10-7 M (M = molarity‚ moles/Liter). The hydronium ions in pure water come from the self-ionization
Premium Acid Acetic acid PH
A carboxylic acid is an organic acid characterized by the presence of at least one carboxyl group. The general formula of a carboxylic acid is R-COOH‚ where R is some monovalent functional group. A carboxyl group (or carboxy) is a functional group consisting of a carbonyl (RR’C=O) and a hydroxyl (R-O-H)‚ which has the formula -C(=O)OH‚ usually written as -COOH or -CO2H. Carboxylic acids are Brønsted-Lowry acids because they are proton (H+) donors. They are the most common type of organic acid. Among
Premium Carboxylic acid Acetic acid
Abundance and Extraction of Metals 1. a Mineral - A mineral is a naturally occurring inorganic solid‚ with a definite chemical composition‚ and an ordered atomic arrangement. b Ore - Deposit in the Earth of valuable metals contained in minerals. c Economic Natural Resource Deposit - This is a natural deposit of something that is economically viable to mine‚ refine and sell. Hence the deposit must be big enough to last for a large period of time‚ so that it will make enough money for the
Premium Aluminium Oxygen Mineral
Experiment #1: Separation of Acid‚ Base‚ and Neutral Compounds from a Solid Mixture by Liquid-Liquid Extraction Introduction Liquid-liquid extraction is a technique used to separate chemical substances in order to purify or identify the various components of a mixture. Flavors‚ spices‚ perfumes‚ and medicines are just some of the everyday things that are extracted from plants and other natural sources [1]. The basic principle used to carry out this separation is the mixing of two liquids that are
Premium Solubility Chemistry Solvent
JUICE EXTRACTION After weighing‚ sugarcane is loaded by hand or crane onto a moving table. The table carries the cane into one or two sets of revolving knives‚ which chop the cane into chips in order to expose the tissue and open the cell structure‚ thus readying the material for efficient extraction of the juice. Frequently‚ knives are followed by a shredder‚ which breaks the chips into shreds for finer cane preparation. The chipped (and shredded) cane then goes through the crusher‚ a set of roller
Premium John Stuart Mill Sugar The Table