Determining the Optimal Temperature and PH of Barley Amylase Abstract The purpose of this experiment was to find the optimal temperature and pH of barley alpha-amylase. I hypothesize that the optimal temperature would be 55 degrees Celsius and the optimal pH would be 5.5. In this experiment‚ the starch is used as a substrate to examine the optimum temperature and pH for the reaction of alpha amylase. It is known that the measuring of disappearance (absorbance) of the substrate starch with iodine
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Effects of pH on fungal amylase activity BI 211 November 25‚ 2011 Introduction In recent years‚ the uses of microorganisms have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries‚ in which demand for them is increasing continuously‚ amylase is also used in
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Abstract: This experimentation was to evaluate absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To
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Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because we assume the more substrate an enzyme gets
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Hypothesis The optimum temperatures of Alcalase and Savinase will be different. Above and below their optimum temperatures activity will decrease. Biological explanation This investigation is designed to look at the effect of temperature on the activity of the proteases Alcalase and Savinase. By the end of it I hope to know the optimum temperature of both proteases. The substrate I am going to use during the experiments is the protein gelatin‚ which is a translucent‚ colourless‚ brittle solid
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of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction temperature of
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The aim of the experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC‚ which is the body temperature‚ where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working. Introduction:Enzymes are made
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How does temperature affect the rate of reaction for Lipase? As the temperature increases‚ so will the rate of enzyme reaction. However‚ as the temperature exceeds the optimum the rate of reaction will decrease. I predict that at temperatures above 70°C the enzyme lipase will become denatured and at temperatures below 10°C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately
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instructions in the cell: to switch genes on and off‚ to make proteins and enzymes Unit 3 Page 1 enzymes DNA is a double strand of polynucleotides The sugar-phosphate backbone is on the outside‚ the bases are on the inside The strand is held together by hydrogen bonds between the bases A only pairs with T. G only pairs with C. ○ This is called complementary base pairing - The two strands run in opposite directions ○ This is called anti-parallel - DNA twists into a double-helix‚ help by more
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IA Report 2011 5/31/2011 Enzyme Activity Experiment Introduction 3 AIM 3 Materials 3 Method 4 Result 5 Table 1: 5 Table 2: 5 Graph 1 6 Discussion 6 Conclusion 8 Bibliography 9 Appendix 1 (Test tube 3- 45 degrees) 9 Appendix 2 (Test tube 1- 5 degrees) 9 Appendix 3 (Test tube 2- 20 degrees) 9 Introduction Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8.3 phenolphthalein will go colourless. Here
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