The Grignard Synthesis of Triphenylmethanol Abstract: Grignard synthesis of triphenylmethanol was achieved by use of the Grignard reagent phenyl magesium brominde. The organometallic grignard reagent was synthesized by use of a reflux apparatus recrystallization techniques. Once synthesized it was used in a Grignard reaction that involved nucleophilic addition to a carbonyl in order to make triphenylmethanol. The final product was solidified and recrystallized and spectral data was
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ineffective. In order to help resolve the crisis‚ antibiotics were found through screenings of soil microorganisms. However‚ soil microorganism’s antibiotics were depleted by the 1960s and their antibiotic effects were unable to be replicated through synthesis. In this experiment‚ the researchers developed numerous methods to cultivate uncultured organisms in their environment. The goal of this experiment was to find antimicrobials in the uncultured soil. Through this successful experiment‚ the researchers
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is associated with lactase persistence is the located upstream of the LCT gene. In this mutation a single nucleotide polymorphism changes a cytosine into a thymine that then can be detected using the polymerase chain reaction technique (Biology 225 lab manual‚ S2017). In this experiment amplification Refractory Mutation System PCR was used to detect this nucleotide change. Two primers were used in two different PCR reactions‚ one to detect the wild type allele and the other to detect the mutant allele
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FOUNDATION IN SCIENCE INTRODUCTION TO PRACTICAL ORGANIC CHEMISTRY – MAKING AND PURIFYING ORGANIC COMPOUNDS Stage 1 – Deciding how much reagent to use Most organic reactions do not go to completion; most reach an equilibrium position with significant quantities of reactants still remaining. In addition‚ separating the desired product from the rest of the reaction mixture‚ and then purifying it‚ will result in the loss of more of the product. The amount of product obtained expressed as a percentage
Free Distillation Sulfuric acid Acetic acid
The structure and synthesis of TPC is outlined in Scheme 1. Briefly‚ the compound 1 was synthesized as per literature procedure. 2 was synthesized by reacting 1-methy pyrazole-4-boronic pinacol ester with 1 by suzuki-coupling‚ the final product TPC was synthesized by treating with malononitrile in the presence of piperidine as a base and ethanol as solvent. The detailed synthesis procedure given in the experimental section. The structure and purity of TPC was unambiguously confirmed by 1H and 13C
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follows a semi microscale technique even a small amount of lost product yields a great loss in the yield 4. These sources of error could be minimized by doing a larger reflux in order to increase the yield. However‚ as the purpose was to simply synthesis SnI4 in order to identify the stable oxidation states (the melting point range requires very little product) it would be advantageous to use the current method. This is due to the smaller quantity of materials needed for this type of reflux‚ thus
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A small amount of the unknown (approximately the tip of the spatula was used for all the pigments) was added into each clean dry test tube. For the first test 1 mL of 3 M of HNO3 was added. As soon as the acid came into with the solid‚ the pigment was observed to dissolve while also turning the solution into a pale clear colour. The same amount was used for the HCl reagent. As soon as the acid touched the pigment it yielded in a clear solution (solid dissolved after a few shakes). When the KI was
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As shown in the balanced chemical reaction in figure 1‚ Felbinac is synthesized from 4-bromophenyl acetic acid‚ phenylboronic acid‚ and 10% Pd/C. Figure 1: The balanced chemical reaction showing the synthesis of felbinac. It functions by inhibiting cyclooxygenase-1‚ which releases prostaglandins that cause pain as a response to injury. In a study conducted by Moore et al.‚ 160 patients were subject to various topical NSAIDS to determine their effectiveness
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TRANSCRIPTION: Transcription is the process of which DNA matches corresponding RNA bases‚ Transcription is located in the Nucleus‚ and the only type of RNA that is involved in Transcription is mRNA‚ and the purpose is so that the code can get out of the Nucleus‚ mRNA is also made through Transcription‚ It also takes information that doesn’t directly make proteins but it helps makes codes for the production of proteins‚ DNA Transcription consist of 4 nucleotide bases‚ Adenine‚ Thymine‚ Cytosine‚ Guanine
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In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used
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