incubating the reaction tubes in a 37°C waterbath for 45 minutes. Finally‚ after the incubation was complete‚ 5 µl of 10x gel loading solution was added to each reaction tube to stop the reaction. Once again‚ the reaction tubes were covered and tapped gently to mix the solution and stored in the refrigerator for gel electrophoresis. Table 6.1 Summary of Restriction Enzyme Digestion
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2.9 Erbium Doped Fiber Laser The EDFLs can operate in several wave-length regions‚ ranging from visible to far infrared. The 1.55 region has attracted the most attention because it coincides with the low-loss region of silica fibers used for optical-communications [14]. The erbium absorption line that best matches with available high power pump diodes is in the 980 regime. At this wave-length‚ the absorption cross section of erbium is so low that an excessive amount of fiber would be required for
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Extraction from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method used for the amplification
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The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
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amount of DNA ⎯ even from the whole genome. This specific sequence can be found through a combination of two different techniques: Agarose Gel Electrophoresis and Hybridization‚ which is known as Southern Blotting. This technique if performed in three phases: (I) prepare the gel and (II) make the blot‚ (III) hybridize and visualize blot. In phase one‚ the gel is prepared in three steps: (1) chemical digestion‚ (2) electrical separation‚ and (3) chemical denaturation. In the first step‚ the DNA sample
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As only six studies have been reported from India‚ the larger population of the country remains uncovered. According to what has been reported till date‚ b3a2 fusion transcript is most prevalent among adults and b2a2 is found to be more prevalent among children. Thus aim of this study was to find‚ that in what proportion BCR-ABL fusion transcript are present in population studied and how it is affecting prognosis of CML patients. For this study 46 patient samples were collected‚ out of which result
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Introduction For this experiment we compared and analyzed various DNA fragments in order to determine if these DNA fragments originated from the same individual. The learning objective for this lab is to gain a better understanding of how DNA fingerprinting works. In this lab the primary function is to determine which DNA fragments match the DNA fragment found on the crime scene. To determine if any of the DNA fragments match the fragment found at the crime scene‚ the DNA fragments must undergo the
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Craig S. Ricard PSY/285 Due: March 11‚ 2012 Experimental Research Recently‚ a group of teachers wanted to determine the effectiveness of using anti-bacterial gels against the spreading of germs from person to person and overall preventing them from getting the cold or flu. In order to see if using anti-bacterial gels prevents getting the flu or a cold an experiment must be performed with the teachers. The experiment must be properly performed by randomly dividing the teachers into groups
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Agarose Gel Electrophoresis. The objective of this lab was to match a criminals DNA to a crime scene using restriction enzymes EcoRI and Pstl with Agarose gel electrophoresis. Restriction enzymes cut DNA at a specific base pair site recognized by the enzyme‚ which then turns one single strand of DNA into many fragmented strands of DNA. EcoRI recognizes and cuts the palindromic base pair sequence GATTC while Pstl recognizes and cuts the palindromic base pair sequence CTGCAG. Agarose gel electrophoreses
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1 Malunggay Extract as an Organic Hand Sanitizing Gel with the Fragrance of Sampaguita Researchers Gozum‚ Ryouji S. Martin‚ Myra G. Cunanan‚ Jastin Angel D. Galang‚ Kenneth M. Bandrang‚ Alyanna L. Bautista‚ Jaspher L. Manzano‚ Jann Rainiel Adviser Mrs. Haydee J. Manalo Proposal Ingredients: ½ cup Aloe Vera 2 teaspoons of Glycerin 1 cup Ethyl Alcohol Malunggay Leaves Sampaguita Flowers Materials: Mortar and Pessel Scraper Bowl Measuring Cups Measuring Spoons
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