Identifying sites of protein synthesis in Chlamydomonas using erythromycin and cyclohexamide as protein synthesis inhibitors. October 16‚ 2009 Introduction: In living cells‚ prokaryotic or eukaryotic‚ the synthesis (construction) of proteins is accomplished by similar machinery. Amino acids‚ ribosomes‚ messenger RNA (mRNA)‚ and transfer RNA (tRNA)‚ are all necessary for the building of functional proteins in a cell. Ribosomes are the site of protein synthesis in a cell‚ and there are two
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Protein synthesis is one of the most fundamental biological processes. To start off‚ a protein is made in a ribosome. There are many cellular mechanisms involved with protein synthesis. Before the process of protein synthesis can be described‚ a person must know what proteins are made out of. There are four basic levels of protein organization. The first is primary structure‚ followed by secondary structure‚ then tertiary structure‚ and the last level is quaternary structure. Once someone understands
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Protein synthesis is the process whereby proteins are produced‚ or synthesized‚ in living things according to "directions" given by DNA (deoxyribonucleic acid) and carried out by RNA (ribonucleic acid) and other proteins. As suggested earlier‚ this is an extraordinarily complex process that we do not attempt to discuss here. Following synthesis‚ proteins fold up into an essentially compact three-dimensional shape‚ which is their tertiary structure. DNA contains the instructions for a cell’s structure
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living creatures‚ work together in a certain process that is crucial to existence: the formation of proteins. Although all species differ from each other in various ways‚ the processes by which proteins are synthesized are the same in all. Protein synthesis is a very complex process. In order to understand the process‚ there some basics that are essential for cells to create the proper proteins. DNA is a very long and double-stranded molecule that contains coding‚ through four nitrogen bases (adenine
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Sarah Khan Bivins-5th April 20th‚ 2013 Protein Synthesis Every day‚ you take in an abundance of different biomolecules; one of them being proteins. Have you ever wondered how proteins are made? They don’t just grow from a tree or fall from the sky‚ they are made through a process called protein synthesis. Protein synthesis is broken up in two two steps: transcription and translation. Transcription starts inside the nucleus when the DNA is unzipped by helicase. Following that‚ the mRNA nucleotides
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Protein Synthesis Worksheet 1. 2. a. Protein synthesis began in the nucleus. b. Chromatin is a diffuse mass located in the nucleus where genetic material is found. c. A gene is a sequence of nucleotides‚ it is important because they form the template and these templates are used for synthesizing proteins. 3. a. Transcription is the first step of protein synthesis it uses a sequence of DNA nucleotides to produce a sequence of RNA nucleotide. b. RNA polymerase bonds to RNA nucleotides together
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During protein digestion‚ a water molecule is added which breaks down the carbonyl-carbon-nitrogen sin the peptide bonds of proteins causing the liberation of amino acids. This process is referred to as proteolysis. Water molecules break down the carbonyl-carbon-nitrogen single bond (peptide bond) that holds single amino acid molecules together (Caroline Ritchie‚ 2013. This process is called hydrolysis and is catalysed by protease. The three main protease enzymes produced during digestion are pepsin
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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The Role of DNA in Protein Synthesis DNA is found in the cells nucleus. It is part of the chromosome of the cell. DNA is the molecule that controls the making of protein. Protein is used for the growth of cells and organisms and also for the repair of cells. Protein is also used as an enzyme to speed up reactions in the body. Because of this DNA is able to control the whole organism. With out DNA and its ability to make protein the cells and eventually the whole organism would die
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lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used included lysozyme‚ protamine sulfate‚ and bovine serum albumin‚ and the three non-protein samples contained either RNA‚ tyrosine‚ and glycylglycylglycine. Standard curves were created to exhibit the linear relationship between the concentration of solute (protein‚ non-protein)
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