a positive result. Body temperature is the optimal temperature for the action of amylase. Enzymes are catalysts for any reactions. They provide an alternative way of lower activation energy to speed up the reaction. When the temperature is low‚ the kinetic energy of substrate and enzyme is low‚ this take long time for both of the substance to bind together so the rate of reaction is low. When the temperature is high‚ the kinetic energy of the substance and enzyme is high too. The substrate and enzyme
Premium Starch Enzyme
Gel electrophoresis is a routine laboratory procedure in biochemical studies that takes advantage of a protein’s amphoteric nature to determine its molecular weight and charge by running the sample through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The
Premium Protein Enzyme Molecular biology
Discussion Although only the extraction of strawberry DNA was performed in this lab‚ this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well‚ and compare it to the steps taken in the strawberry protocol. As described in the procedure using strawberries‚ the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective
Premium Protein DNA Cell
Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
Premium Cellular respiration Adenosine triphosphate Citric acid cycle
Introduction DNA‚ or deoxyribonucleic acid‚ is a double stranded helical structure used to store genetic information in cellular organisms. DNA usually consists of two strands made up of nucleotides‚ each with a backbone of repeating units of phosphate groups and the sugar‚ deoxyribose‚ bonded by phosphodiester linkages. Since the deoxyribose has a specific orientation‚ DNA molecules have directionality so that DNA sequences are read 5’ to 3’. The 5’ end of DNA is characterized by the deoxyribose
Premium DNA Gene Genetics
Tan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube‚ spatula‚ glass rod‚ dropper‚ beaker‚ test tube rack‚ skewer. Materials : - 1 egg‚ meat tenderizer‚ salt‚ water ‚ soap‚ isopropyl alcohol 91%‚ pineapple juice. Variables : Manipulated Variable : Responding
Premium Egg DNA Cell
Destiny Thomas Biology October 19th 2012 Period: 3rd Destiny Thomas October 19th 2012 Biology Period: 3rd Introduction In our experiment we used only the red colored life savers. They all had about the same mass. We used 9 life savers. In our experiment we had to dissolve our life savers. The concept of dissolving is to place the
Premium Chemistry Life Heat
Gel electrophoresis is a procedure which sorts molecules based on size and charge. The gel in gel electrophoresis refers to the object that separates the molecules. Electrophoreses refers to the force that is used to move the molecules through the gel. There are 2 stages to gel electrophoresis‚ separation and visualization. During separation the gel matrix is placed in an electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel
Premium Gel electrophoresis Protein Power
tubes 2) Boiling water bath 3) Microscope 4) Slides and cover slips 5) Pasteur pipettes MATERIALS: 1) pH test strips 2) Benedict’s reagent PROCEDURES: Collect the urine samples in a container. Make sure to not spill anywhere in the lab. Complete the tests and all results/ observation are in a proper table. (A) Observation of urine 1) The urine is sniffed and the description of the urine is described. Terminologies like slightly nutty‚ sweet/ fruity or pungent can be used
Premium Urine
The investigation on the average mass of DNA with the mass of banana‚ strawberry and kiwi. Purpose: The purpose of this lab is to investigate the comparison between the amount of DNA per gram of fruit that can be extracted from a banana‚ strawberry and kiwi and to determine which one has more DNA. Hypothesis: The banana genome contains 837 MBPs and the strawberry genome contains 206 MBPs and the kiwi genome contains 128 MBPs. This states that there are more base pairs in a banana genome
Premium Fruit DNA