Radioisotopic labels would be used in experiments to identify semi-conservative replication in prokaryotes. Because we anticipated that a labeled DNA would have different density with unlabeled‚ which means‚ by analyzing the different density of DNAs‚ we can determine which of DNA is labeled‚ half-labeled or unlabeled. To this end‚ I will use c13 label the bacteria and abruptly change carbon source with C12. Then I will collect four samples in different time and analyze the results from centrifugal
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Bacteriophage A bacteriograph is any of a group of viruses that infect specific bacteria‚ usually causing their disintegration or dissolution. They are made of an outer protein coat or capsid that encloses the genetic material. They inject their genetic material into the bacterium following infection. When the strain is viruilent‚ all the synthesis of the host’s DNA‚ RNA and proteins ceases. The phage genome is then used to direct the synthesis of
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Value of the data • This is the first draft genome of Trametes villosa‚ a tropical white-rot Basidiomycota from the semiarid region of Brazil‚ promising for its production of ligninolytic enzymes. • T. villosa isolate CCMB561 is a good producer of lignin peroxidase‚ manganese peroxidase‚ and laccase‚ enzymes considered key for lignin degradation‚ providing a major advantage for its use in bioenergy research. • The draft genome will accelerate functional genomics research‚ helping to understand the
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Through this experiment‚ Egeria densa was observed using a microscope. The task was to observe and identify the different types of cell‚ cytoplasmic streaming‚ and plasmolysis of Egeria densa. First‚ the microscope was examined and investigated to master the use of the equipment. A microscope slide grid which was on the slide glass was required to be seen clearly using 4x‚ 10x and 30x. During the latter part of the experiment‚ the Egeria densa was observed using the microscope to understand the
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Executive summary This experiment has been conducted to accumulate data on the growth of Escherichia coli (E. coli) and to monitor how it grows under certain conditions. It has been demonstrated that the levels of glucose and dissolved oxygen were found to affect the rate of growth of E. coli proportionally with a lack of oxygen resulting in the lowering of the pH. In this experiment the growth of E. coli was studied at constant temperature (37 0C) at which it grows ideally. Experimental results
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OBJECTIVE The purpose of this lab was to troubleshoot causes related to PCR components and develop an experiment that would test if the Taq amount is suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your
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FISH FISH stands for Fluorescence in situ hybridization. FISH is a technique in the lab used to see where a DNA sequence or certain gene is located in an individual’s genome. This allows scientists to look for genetic conditions caused by alterations in chromosomes. FISH can be used to find distinct features in DNA for genetic counseling‚ species identification‚ and medicine. It can also be used to find certain RNA points in cells and tissue samples. FISH is used on blood samples or any other cell
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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Isolation of plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal
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Experiment title: Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page
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