AND BIOTECHNOLOGY GENE CLONING . NAME: KANENDA. Ally. O REG #: 2013-04-02841 DEGREE PROGRAM: MOLECURAL BIOLOGY AND BIOTECHNOLOGY COURSE INSTRUCTOR: Dr. ROSE MASALU Gene cloning: Gene cloning is the process of inserting a gene of importance into a recipient organism‚ usually a bacterium‚ in order to replicate that gene many times and mass-produce a useful gene product. The recipient organism is called the host‚ and the material used to introduce the gene‚ usually a plasmid or bacteriophage‚
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Gene Cloning Clone: A group of identical cells or organisms To clone a gene: 1) Insert the gene into a vector that can carry the into a host cell 2) Ensure that it will replicate there 3) Insertion is carried out by cutting the vector and the DNA to be inserted with the same restriction endonuclease to ensure that both have the same "sticky ends" Vector for cloning the bacteria come from two major types: (1) Plasmids‚ and (2) Phages Recombinant DNA Technology A) It was developed in
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Alan Holmes Jr. Ms. Maureen Cunningham ENG 090‚ 4501 26 January 2011 What is expression? When I sit back and actually think about all the things that people do to express their thoughts‚ feelings‚ and beliefs‚ it really amazes me at the things people will do. It all starts when you are a young kid and you just have to tell everyone how you feel or what you saw. When you are young you will say exactly what is on your mind even if it is not in the best interest of others. Even though it seems
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Genes Review 1) Selective breeding – chosen based on what can be seen and detected (favourable phenotype) Genetic engineering – selected based on genes 2) - DNA are found in nucleus (where most DNA are found)‚ mitochondria‚ chloroplast 3) - Translation requires ribosome‚ mRNA‚ tRNA (anticodon) and amino acids - DNA template‚ polymerase for transcription 4) Mitosis generates daughter cells that are identical Meiosis – homologous recombination 5) DNA coding sequence: 5` GGGCCCTTTAAA
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Cloning of plasmid pUC19 in E.coli bacteria Introduction One aspect of the DNA cloning experiments that is carefully considered is the selection of cloning vectors. A variety of vectors have been created‚ each being suitable for a particular use. One common vector used in laboratories is a plasmid called pUC19. It is 2686 base pairs long and possesses an origin of replication which allows the production of over 100 copies in a competent E.coli cell. It possesses a multiple cloning site (MCS)
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expressions The Expressions Company began in 1988 as The Expressions Card and Stationery Shop‚ offering greeting cards and other paper products exclusively distributed by Sterling Paper. Two decades later‚ The Expressions store has grown to over 40 branches nationwide and has expanded its merchandise to include complete lines of school and office supplies‚ gift and novelty items‚ computer supplies and reading materials‚ living up to its current positioning as "Your affordable school and office
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Lecture 10 Gene cloning BIOLOGY Campbell‚ Reece and Mitchell Chapter 20 Gene cloning technology Also known as: Genetic engineering or Genetic manipulation (GM) technology – implies precision engineering being applied to DNA molecules Recombinant DNA technology - implies that new combinations of DNA molecules can be made I.e. “recombinant” DNA molecules Overview of Genetic Engineering procedure 1. Making recombinant DNA molecules that can replicate in bacterial cells Genetic engineering
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and the assurance to a higher standard of future for their children. However‚ this may not be accurate for all families. One or both of the parents have chosen to work overseas because of the worsening economic condition in the Philippines. From genes to bequests‚ parents have important influences on the income‚ health and general living standards of their children as adults (Ermisch‚ 2003). Parents either working within the country or overseas influence their children in countless ways. They create
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transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism. A gene is a piece of DNA that
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MANAGEMENT PROJECT REPORT Letter of Transmittal: Sir Jawad Bhatti‚ Subject: Operations Management Report on Lux Soap Respected Mr. Jawad Bhatti‚ We present to you our Report on the operation management of Lux‚ a part of our term report. The preparation of this term report was a challenging task but it provided us with the much-important knowledge about the operations of Lux soap. We have put in great efforts in the preparation of this report and have tried hard enough to match the requirements
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