EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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Armadillium vulgare taxis response to different pH levels. Abstract The terrestrial isopod‚ Armadillium vulgare is commonly referred to as a slater or pill bug. Since transitioning from the sea to land and originally colonizing in Mediterranean regions‚ it has adapted throughout evolution to inhabit local microhabitats. The pill bug is bound by several parameters and also has specific requirements that need to be met for optimal biological functioning. As such‚ behavioural and physiological
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The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing
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digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7)‚ and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica‚ n.d. Web. 10 Dec. 2012. Research Question: Does changing the pH of pepsin affect the ability to break down the protein (mass of jello). Variables: independent- Ph level dependent- The mass of the jello
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Using Buffers Gino A. Romeo‚ Jr.‚ Ph.D. Version 42-0134-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
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Effects of pH on fungal amylase activity BI 211 November 25‚ 2011 Introduction In recent years‚ the uses of microorganisms have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries‚ in which demand for them is increasing continuously‚ amylase is also used in
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pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
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Myrtle Freedom under threat: PH‚ 2rd deadliest country for Journalists Journalism has long been a high-risk profession‚ many journalists and reporters have put their lives on the line for a story. Journalists often have to go through extreme measures just to get information. Intensive research‚ live coverage and interviews are being demanded more and more by the public and in order to get such vital information‚ being on the scene is necessary. According to Committee to Protect Journalists (CPJ)
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I. Introduction The purpose of this experiment is to determine the pH values of acids‚ bases‚ and buffers of distilled water and 10.0 buffer using measured concentrations of Sodium hydroxide (NaOH) and/or Hydrochloric acid (HCl). Acid is a compound typically having a bitter taste and capable of nullifying alkalis and releases hydrogen ion when added to a solution‚ or containing an atom that can accept a pair of electrons from a base (McKinley‚ Dean O’Loughlin‚ & Stouter Bidle‚ 2016). Bases are water-soluble
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The Effect of pH on a Food Preservative September 18‚ 2012 Objective: To study the affect of pH on a food preservative. Chemical Equation: C6H5COONa + HCL ------ C6H5COOH + NaCl Procedure: This experiment was started with a clear solution of sodium benzoate and HCl was added to it‚ ultimately producing benzoic acid. First‚ .3395 g of sodium benzoate was weighed‚ then it was dissolved in water‚ causing it to disassociate into ions. Next‚ 3M of HCl were added drop wise to the solution
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