Sonia St James Investigation: Factors that affect the outcome of enzyme activity Introduction In this project I will monitor the rate of activity of Catalase. Catalase is an Enzyme which in the right conditions catalyses the decomposition of Hydrogen Peroxide into water and oxygen; 2H2O2 + Catalase >>> 2H2O + O2 Catalase is found in all cells and protects them from Hydrogen Peroxide which is a dangerous waste product that needs to be eliminated. Without Catalase living things could not survive
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Enzymes lower the activation energy of chemical reactions but they themselves are not consumed or altered when doing so. These catalysts work best at optimum temperatures and pH’s. The temperature and pH at which the reaction occurs the quickest is the ideal condition for the enzymatic reaction. Alpha amylase converts starch into glucose and when starch is combined with I2KI indicator a dark purple solution forms. As the enzyme breaks down the starch the absorbency will decrease. The absorbency
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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determining the effects of pH on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient showing that as the percentage transmission will decrease with an increasing pH. This happens because
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Diffusion continues until it reaches equilibrium. Osmosis is similar to Diffusion but it’s the process in which water moves across a semi-permeable membrane and goes to the higher concentration of solute.1 2.2. Important factors to Osmosis and Diffusion include Temperature‚ Concentration and Surface area to volume ratio. Temperature can affect the rate in which a solute dissolves in a solution‚ higher temperature faster rate of Osmosis. This is caused by the molecules movements being faster
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Biology Coursework: Preliminary Work 8/12/2013 Intro I am going to investigate a factor that affects the rate of enzyme activity What are enzymes and how do they work? Enzymes are biological catalysts - catalysts are substances that increase the rate of chemical reactions without being used up. Enzymes are also proteins and thus have a specific shape. They are therefore specific in the reactions that they catalyse - one enzyme will react with molecules
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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WHAT IS JUNK FOOD ? The term “junk food” is used to describe food that is low in nutritional value‚ with a comparatively high caloric value. Many people try to avoid or limit such food in their diets‚ out of concerns that it is not healthy‚ despite the fact that numerous food manufacturers produce a range of products which could be considered junk. Nutritionists‚ doctors‚ and other health advocates often work to educate people about junk food‚ encouraging them to eat well balanced diets which contain
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retains the solids and contaminants in the water. The membrane is the most important part of the system. It very hard to located problems in the membrane. In addition‚ if the membrane is not in perfect condition‚ the system is not working. Reverse osmosis is a kind of filtration. Filtration is an oldest process that consists in the water passing through sand‚ gravel or membrane. Thus prevent the solids and contaminates passing for the other side. The pores of the filters need to be small. The smaller
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Biochem. J. (1995) 305‚ 17-20 (Printed in Great Britain) 17 RESEARCH COMMUNICATION The effect of low temperatures Nicole MORE‚ Roy M. DANIEL* and Helen H. PETACH on enzyme activity Thermophile Research Unit‚ University of Waikato‚ Private Bag 3105‚ Hamilton 2001‚ New Zealand The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain ANI and f‚- enzymes‚ glucosidase from Caldocellum saccharolyticum expressed in Escherichia
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