oligosaccharides‚ and polysaccharides are theclassifications of carbohydrates. This experiment involves the carbohydrates starch‚ glucose‚ fructose‚ maltose‚ xylose‚ and sucrose. These carbohydrates are the standards to be used in determining the unknown sample with the help of the Barfoed’s test‚ Benedict’s test‚ Bial’s Orcinol test‚ Seliwanoff’s test‚ and Iodine test. Introduction Carbohydrates are the single most abundant class of organic molecules found in nature. Along with proteins and
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this experiment‚ the osmolality given by the machine was equal to the molality. 1) Finding the identity of a unknown substance Molar Mass of Possible unknown substances (g/mol): * Glycine: 75.067 * Glucose: 180.155 * Mannitol: 182.171 * Sucrose: 342.26 * Lactose: 360.312 Results: Table 1.1: Table showing the results for the osmolality. 200mg of the unknown was dissolved with 10mL of doubly deionized water. A 0.2mL sample was taken and placed in the Osmometer. Trial
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to produce sodium chloride‚ water and carbon dioxide. Experimental Procedure: First the student will take the weight of a clean dry beaker and record the data. Next ass .15g of the first unknown substance. From there‚ the student will add 50ml of water to the beaker then dissolve the .15g of the first unknown substance into the water. Once the substance has dissolved‚ the student will add 10 drops of bromocresol (indicator) into the beaker. After the student will fill the buret all the way up with
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Abstract Carbohydrates are important in metabolic processes for everyday physical and chemical actions. The carbohydrate‚ glucose‚ is a key component in generating adenosine triphosphate‚ also known as ATP. In order to analyze unknown glucose levels‚ a DNS assay was performed. By using 2-hydroxy-3‚5-dinitrobenzoic acid to oxidize the aldehyde group on the carbohydrate‚ the reducing end of glucose increases in absorbance of 540 nm. Using a UV spectrophotometer‚ the concentration was calculated
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Lehman’s Operational Organic Chemistry Lab Manual. (Pgs. 31-32). 2) See page 36 of Lehman’s Operational Organic Chemistry Lab Manual Results To find out the identification of the unknown‚ 3 separate trials had to be performed to the unknown‚ some standards and a mixture of 50/50 of the unknown and the standard‚ or in the case of trial 3‚ both standards of aspirin & acetylsalicylic acid were combined in a 50/50 mixture. (See Table 1 below). * = Standards. In each trial each separate
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Determination of Ka of Weak Acids Purpose: The purpose of this lab is to find the strength of weak acids by determining the equilibrium constants for their ionization reactions in water. Is to use their measured pH values to calculate the pKa for the two unknown weak acids thus determining their identities. Hypothesis: If we neutralize a solution that contains a weak acid by adding a strong base to the solution‚ then the ions will be isolated and thus finding the concentrations of each species by measuring
Free PH Sodium hydroxide Acid
Objective:- The aim of this experiment was to find the molar mass of unknown volatile liquid under known temperature and pressure. Introduction Molar mass or gram molar mass is the mass of the substance under consideration without distinguishing whether a substance is an element ‚ a molecule or an ionic compound. These elements‚ molecules or ionic compound could be solid gas or liquid. The molar mass of gas or vapor could be measured if the gases obey the law of Ideal Gas . The mass of
Free Gas
ingredients. During the experiment‚ four different substances (cooking ingredients) were tested on‚ plus the substance on Smeddy‚ with three different liquids. The five substances tested on were baking powder‚ baking soda‚ cornstarch‚ sugar‚ and Unknown A (the substance Smeddy had). The liquids used to analyze the chemical and physical changes on the substances were water (H2O)‚ vinegar‚ and an iodine solution. In a spot plate‚ a small amount of each substance was put into three separate spots and
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in separating components of a mixture is paper chromatography. We use paper chromatography to separate mixtures and isolate there components to collect them individually. In this experiment‚ we will use acid- base indicators to help determine the unknown mixture. Acid- base indicators will change in color when the presence of pHs rise and fall when acids or bases are added to a solution. After setting strips of paper into a flask with the eluding solvent and waiting 45-60 minutes‚ we will set the
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between a life threatening diseases to a creation of certain antibiotic. Understanding the principals of living microbes and identifying my unknown bacteria through numerous biochemical and metabolism tests‚ with the outmost confidence‚ Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the experiments and comparing them to the other six known bacteria: Micrococcus luteus‚ Staphylococcus
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