Lab 5 –Cell Structure and Staining using Microscopy Instructions: Please download this MSWord document to your computer and answer the questions as asked. Then save the document and upload it to Bb using the Assignment feature provided. This assignment is worth a total of 100 points – there are 20 questions worth 5 points each. NAME Buket Rembert In Lab 3 you were introduced to microscopy. In this lab you will be adding to that experience by reviewing the differences in cell structure for Prokaryotes
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Abstract This experiment was carried out to perform staining methods on microbes‚ explain the mechanisms of staining‚ namely‚ simple staining‚ and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify
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Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells
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Kevina Smith Lab 1: Microscopy and the Metric System Part A: Microscopy Purpose The purpose of this experiment was to learn how to use a microscope correctly and perform wet mount slides accurately‚ thus becoming more familiar with the microscope. Hypothesis It would be hard to use the microscope without any kind of previous training and the parts of the microscope and their functions must be learned in order to use it properly. Materials & Methods Materials: 1. Filter
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Paper submission on electron microscopy professor: Ernesto Suarez by ananthalakshmi adapa University of Hartford What is a electron microscopy? An electron microscope (EM) is a type of microscope that uses an electron beam to illuminate a specimen and produce a magnified image. An EM has greater resolving power than a light microscope and can reveal the structure of smaller objects because electrons have wavelengths about
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BIO Microscopy‚ Mitosis‚ and Motility Lab Discussion Questions In the Microscopy‚ Mitosis‚ and Motility lab‚ the class used the Compound Microscope. The Compound Microscope helped examine the prokaryotic and eukaryotic cells‚ observe the different stages of mitosis‚ and compare modes of motility used by protists. The light compound microscope works with the aid of lenses such as the eyepiece. These lenses help keep the focus of the light on the eye. The fine and course adjustments also assist with
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Microscopy Cell are very Small Metabolic requirements set upper limits on the size of cells The surface area to volume ratio of a cell is critical As the surface area increases by a factor of n2‚ the volume increases by a factor of n3 Small cells have a greater surface area relative to volume Figure 6.7 Surface area increases while total volume remains constant 5 1 1 Total surface area [sum of the surface areas (height width) of all box sides number of boxes] 6 150 750 Total
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Microscopy and the Metric System Margaret E. Vorndam‚ M.S. Version 42-0090-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports
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