PRACTICAL 3 QUESTIONS PART 1 Q 1. What is the diameter of the field of view of your microscope using the x10 objective (i.e. at a magnification of x100)? The diameter of the field of view using the x10 objective is 1800 µm. Q 2. What is the diameter of the field of view of your microscope using the x40 objective (i.e. at a magnification of x400)? The diameter of the field of view using the x40 objective is 400 µm. Q 3. What is the ratio of magnification between the x10 and x40 objectives?
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available to the cell biologist that aid in their study. The light microscope is to this day perhaps the single most important instrument used in Cell Biology. It is used under bright field conditions to study the organization of cells in fixed and stained sections of tissues. With phase contrast optics it is possible to monitor the movements of living cells and to observe changes in their subcellular organization. The light microscope may also be used to monitor certain operations such as cell fractionation
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the microscope stage and observed the cells in it at low and high magnification‚ drawing a diagram for both of them. At the end I have taken in consideration all my observation and come to the conclusion that‚ indeed‚ cells are existent in both living beings but their not completely alike. Background To be able to complete this lab a person should know the animal cell and the plant cell structure to the able to identify and label the cell components. Knowing the components of a microscope and
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components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also
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differentiating shape and structure through utilization of microscope. Procedure – Instructions followed as per procedure‚ specimens viewed through v-scope‚ using 10x‚ 20x‚ and 40x stained wet-mount. Observations compared amongst one another from cd-rom. Spirillum bacteria Bacillus Cheek Swab Yeast Analysis – Using the virtual microscope‚ the images seemed to be more clear and concise. Using the light microscope again for another experiment has increased my confidence
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by exploring and discovering the microscopic world. At the start of his career‚ Skip Palenik went to work at the McCrone Research Institute‚ his mentor Walter McCrone’s lab‚ who was one of the only scientists who remained dedicated to the use of microscopes. During Palenik’s time at McCrone‚ he learned logical deduction based off a game in which the goal is to correctly identify the unknown substance‚ known as the U.F.O. game‚ as well as learning that every particle is a clue to its origin. Since his
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BME 42-620 Engineering Molecular Cell Biology Lecture 06: Basics of Cell Biology Literature Reading Methods of Cell Biology (I): Imaging Basics of the Diffusion Theory BME42-620 Lecture 06‚ September 15‚ 2011 1 A Case Study: Malaria & Artemisinin y • Malaria is a mosquito-borne infectious disease caused by y parasite Plasmodia. • 225 million cases each year; 1 illi h million deaths in 2010 (World malaria report). • 4000 recipes • 380 extracts from 200 herbs • Compound
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The onion epidermal cell is transparent with a simple structure so it is a suitable cell for studying the effect of water loss on cells (2) Some specimens can be viewed directly underneath the microscope but putting a drop of water on the specimen can improve how the structures appear under microscope and also prevent the specimen from drying out on the slide (2). Adding water to the specimen is called wet mount. The liquid used in wet mount fills the space between the slides to support the specimen
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macroscopically (in a such a way as to be large enough to be visible to the naked eye; in a way which is comprehensive or is concerned with large units) and microscopically (Too small to be seen by the unaided eye but large enough to be studied under a microscope). When analyzing the hair macroscopically you should look at the length‚ color‚ sheen‚ whether it’s wavy or straight‚ and the presence or absence of follicle tissue or
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POB II: Exam II Review Chapter 6: tour of the cell Microscopy: • Microscopes provide a window to the cell. • Most important tool in cytology • Resolution has improved understanding of cells. o Resolving power – the smallest distance between two objects that allows them to be seen as distinct objects. o Resolving power of the human eye is 0.1 mm • Light microscope – uses glass lenses and visible light to form a magnified image of an object. o Resolving power of about 100 nm (.0002 mm)
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