consisting of two or more windings which are linked with a common magnetic field used for the distribution of electrical power from one voltage level to another. Some of the main parts of the transformer are; the windings‚ tank and insulation (solid or liquid). Transformers normally have an operating temperature range of 60-90oC and an operation lifetime of 40 years [8]. These service temperatures cause gradual degradation of the oil and paper insulation thereby producing degradation by-products such
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Table of Contents Title Page No. 1.0 Introduction 1 2.0 Sample Treatment 3 3.0 Extraction 4 4.1 Solid-Phase Extraction 4.0 Separation 6 5.2 High Performance Liquid Chromatography (HPLC) 5.3 Thin Layer Chromatography (TLC) 5.0 Detection and Identification 7 6.4 UV-Vis Spectrophotometry 6.5 Mass Spectrometry 6.6 Nuclear Magnetic Resonance Spectrometry 6.0 Health Benefits of Daidzein
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Experiment 5 Analysis of Plant Pigment Purpose: Chromatography is used for separation. In this case the separation of plant pigments. After placing the plant sample in the column the separation will be easy to see and the samples will be analyzed using VIS spectrophotometry. This is used to isolate and identify the different lipids causing the different pigmentations. Introduction: Lipids are chemically diverse substances found in cells. Lipids are water-insoluble‚ structural components
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CHROMOTOGRAPHY Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle that they all have a stationary phase (a solid or a liquid supported on a solid) and a mobile phase where liquid or a gas is involved. The mobile phase flows through the stationary phase and carries the components of the mixtures with it. Different components travel at different rates. In paper chromatography‚ the stationary phase is a very uniform
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diagram of the inner workings of the instrument can be seen in figure one below. First‚ a sample is injected into the injection loop with the use of a syringe. After the injection is complete‚ the lever of the injection loop is pulled down to create a high pressure pump that pushes the sample into the mobile phase of the instrument. This mobile phase‚ which for this experiment will consist of water and methanol‚ transports the samples toward the packed column where it will leave the mobile phase and
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Liquid Chromatography – Laboratory #18 Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes‚ and then we separate the flavorings and citric acids. Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid
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The average University student has many tasks to accomplish and much studying to do while combating the onslaught of sleep. Students at the University of the West Indies Mona are no different and recommended amongst themselves a highly rated and popular health supplement “Yeast-Vite” [8]. Yeast-Vite is a health supplement pill which helps people fight fatigue and improve alertness. The active ingredients in Yeast-Vite are caffeine‚ vitamin B1‚ vitamin B2‚ vitamin B3. The other ingredients are:
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and fatty acids. • Spectroscopic Techniques: Structural determination of an Organic compound by using UV & Visible ‚ IR ‚ NMR ‚ MASS techniques. • Chromatographic Techniques: Paper‚ Column ‚ Gas-Liquid chromatography ‚ High performance liquid chromatography etc. Computational Skills: Operating Systems : Win XP‚ 2000‚ 98‚ Languages : C‚ C++‚ Packages : Ms-Office. Soft Skills: 1. Good communication
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HPLC Theory: System Suitability Parameters High performance liquid chromatography is defined as a separation of mixtures of compounds due to differences in their distribution equilibrium between two phases‚ the stationary phase packed inside columns and the mobile phase‚ delivered through the columns by high pressure pumps. Components whose distribution into the stationary phase is higher‚ are retained longer‚ and get separated from those with lower distribution into the stationary phase. The
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(Enclosure No. 4 to DepEd Memorandum No. ___‚ s. 2012) Format of Research Paper Investigatory papers that were reviewed by the national SRCs in the past years were found to have inadequacies in the content particularly in the areas cited below. These rules are found in the Guidelines (http://www.societyforscience.org/isef/rulesandguidelines) and in the Student Handbook (http://www.societyforscience.org/document.doc?id=12). The description of the research plan and the sample abstracts are provided
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