Activity 1.1.5 ELISA 1. Explain why the antibodies allow scientists to target and identify specific disease agents. The antibodies are specific to the antigen they destroy. 2. Why is the secondary antibody used in the ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate? The enzyme catalyzes the oxidation of the substrate and turns the solution blue. 3. Disease samples from two patients are collected and subjected to serial dilutions before
Premium Antibody Immune system Immunology
serum albumin and bovine serum albumin have epitopes in common? Explain. 13. What is the process resulting in antigen and antibody moving toward each other? Enzyme-Linked Immunosorbent Assay 14. In the “sandwich” analogy of the direct ELISA‚ what is the
Premium ELISA Antibody Western blot
IMMUNOSORBANT ASSAY (ELISA) ■ PRINCIPLE ■ Antigen and antibody reaction are highly specific. ■ Antibody’s FC portion can be attached with enzyme. ■ Specific antigen & antibody reactions with enzyme can be detected by the addition of the substrate specific to the enzyme. ■ Magnitude of antigen and antibody present in the reaction seen with the intensity of colors cab be read by ELISA reader. [pic] Types of ELISA ■ Direct ELISA ■ Indirect ELISA ■ Sandwich
Premium Polymerase chain reaction DNA ELISA
Review Sheet Exercise 12 Serological Testing NAME Kali Rothfuss LAB DATE/TIME 6/22/11 Positive and Negative Controls 1. Why are there a number of washing steps in serological tests? The are a number of steps needed in order to remove any non specific binding that may have occurred. 2. Describe how you would know that you had a “false positive” result. What does this mean for the rest of your results? A positive result with a negative control indicates a “false positive”
Premium ELISA Antibody Western blot
agent‚ to positive serum‚ containing antibodies against that infecting agent‚ is called A) complement fixation. B) RIA. C) ELISA. D) seroconversion. 4. The serology test that may show the antigen-antibody complex as yellow-green under the microscope while using an ultraviolet light is known as the A) fluorescent antibody test. C) AIDS test. B) ELISA test. D) radioimmunoassay. 5. Immunological tests may determine the presence of A) antigen or antibody. C) only antigen. B) neither
Premium Immune system Immunology Antibody
differential solubility characteristics of plasma proteins [3]. Figure 1 Albumin [3] There are several biochemical applications that BSA has been used. The most well-known applications are ELISAs (Enzyme-Linked Immunosorbent Assay)‚ immunoblots‚ and immunohistochemistry [3]. Apparatus and Material Bradford Reagent‚ bovine serum albumin (BSA)‚ distilled water‚ apple juice sample‚ unknown sample of A‚ unknown sample of B‚ 8 test tubes‚
Premium Serum albumin ELISA
Audit Proposal Do Private Laboratories provide HIV/AIDS Counselling and Testing? Nigeria as a country has a population of 167 million people (1) while her HIV prevalence rate is 3.4 %; an approximately 3.4million people are living with HIV/AIDS. This number represents the second highest HIV burden in the world second only to South Africa (2). The Government of Nigeria has intervened and put in place different measures to reduce this incidence to its barest minimum amongst which is the National
Premium AIDS HIV/AIDS ELISA
was used for hemagglutination assay. The concentration of total soluble protein was determined by the Bradford method (1976).Further the quantity of recSRL expressed in transgenic cotton plants were determined by enzyme linked immunosorbent assay (ELISA). 3.6.10. Western
Premium Molecular biology Western blot Southern blot
Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
Premium Serum albumin ELISA Multiplication
MedMira Case Course: Marketing Planning What changes in the environment made it possible to consider the launch of an OTC Aids Test? Be specific. MedMira is known by developing and manufacturing quality diagnostics to prevent and control the spread of infectious diseases. All tests used a flow-through membrane technology and were fast and easy to use. Medmira was distributing rapid HIV tests in Canada‚ United States‚ South Africa‚ Latin America and China‚ and selling them to hospitals
Premium Infectious disease HIV/AIDS Western blot